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In Vivo Evaluation of Commercially Available Gel-Type Polyethylene Glycol Membrane for Carrier of Recombinant Human Bone Morphogenetic Protein-2

Authors
 Ji-Woong Jang  ;  Jung-Seok Lee  ;  Ui-Won Jung  ;  Chang-Sung Kim  ;  Kyoo-Sung Cho 
Citation
 JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, Vol.75(297) : 1-13, 2017 
Journal Title
 JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY 
ISSN
 0278-2391 
Issue Date
2017
MeSH
Animals ; Bone Development/drug effects ; Bone Morphogenetic Protein 2/administration & dosage* ; Drug Carriers/administration & dosage* ; Gels/administration & dosage ; Male ; Membranes, Artificial ; Polyethylene Glycols/administration & dosage* ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins/administration & dosage ; Skull/diagnostic imaging ; Skull/drug effects ; Skull/growth & development ; Skull/injuries* ; Transforming Growth Factor beta/administration & dosage* ; X-Ray Microtomography
Abstract
PURPOSE: This study evaluated a commercially available, 3-dimensional gel-type polyethylene glycol (PEG) membrane as a carrier for recombinant human bone morphogenetic protein-2 (rhBMP-2) using a rat calvarial defect model. Another gel-type carrier, fibrin-fibronectin system (FFS), was used as a positive control. MATERIALS AND METHODS: Critical-size defects were made in the rat calvarium, which were allocated to 1 of 10 groups comprising 2 healing periods and biomaterial conditions: 1) sham control, 2) FFS only, 3) FFS plus BMP-2, 4) PEG only, and 5) PEG plus BMP-2. Radiographic and histologic analyses were performed at 2 and 8 weeks after surgery. RESULTS: After 2 weeks, some parts of the FFS were biodegraded and extensive cellular infiltration was observed at sites that received FFS or FFS plus BMP-2. The PEG membrane retained its augmented volume without cellular infiltration at sites that received PEG or PEG plus BMP-2. After 8 weeks, the FFS was completely degraded and replaced by new bone and connective tissues. In contrast, the volume of residual PEG was similar to that at 2 weeks, with slight cellular infiltration. In particular, there was progressive bone regeneration around micro-cracks and resorbed outer surface in the PEG + BMP-2 group. Although the PEG + BMP-2 group showed increased area and percentage of new bone, there was no statistical relevance after 2 and 8 weeks in histomorphometric analyses. However, the appearance of the healing differed (with new bone formation along micro-cracks in the PEG + BMP-2 group), and further studies with longer healing periods are needed to draw conclusions about clinical applications. CONCLUSION: Evidence of mechanical stability and new bone formation along micro-cracks when using PEG plus BMP-2 might support the PEG membrane as a candidate carrier material for rhBMP-2.
Full Text
http://www.sciencedirect.com/science/article/pii/S0278239116301495
DOI
10.1016/j.joms.2016.05.004
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/154095
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