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Simultaneous sentinel lymph node computed tomography and locoregional chemotherapy for lymph node metastasis in rabbit using an iodine-docetaxel emulsion

 Honsoul Kim  ;  Eun-Ji Jang  ;  Sang Kyum Kim  ;  Woo Jin Hyung  ;  Dong Kyu Choi  ;  Soo-Jeong Lim  ;  Joon Seok Lim 
 Oncotarget, Vol.8(16) : 27177-27188, 2017 
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Issue Date
Animals ; Disease Models, Animal ; Drug Stability ; Emulsions*/chemistry ; Iodine*/chemistry ; Lymphatic Metastasis ; Male ; Neoplasm Staging ; Neoplasms/diagnostic imaging* ; Neoplasms/drug therapy ; Neoplasms/pathology* ; Particle Size ; Rabbits ; Sentinel Lymph Node/diagnostic imaging* ; Sentinel Lymph Node/pathology* ; Taxoids/administration & dosage* ; Tomography, X-Ray Computed*/methods ; Treatment Outcome
computed tomography (CT) ; iodine-docetaxel emulsion ; locoregional chemotherapy ; lymph node metastasis ; sentinel lymph node
PURPOSE: A sentinel lymph node (SLN) tracer can gain multi-functionality by combining it with additional components. We developed a SLN tracer consisting of iodine and docetaxel and applied it as a theragnostic nanoparticle to simultaneously perform SLN computed tomography (CT) lymphography and locoregional chemotherapy of the draining lymphatic system. RESULTS: Docetaxel could be loaded in iodine emulsions at a drug-to-surfactant weight ratio as high as that in the drug formulation Taxotere®. The particle size and drug concentration were stable during storage for up to 3 months in optimized nanoemulsions. Popliteal LN enhancement on CT was observed in all healthy rabbits (n=3) and VX2 tumor-implanted rabbits (n=6) 12 hours after injection. The rate of SLN metastasis was significantly lower in the treatment group (29.4%, 5/17) than in the non-treatment group (70.6%, 12/17) (P=0.038). MATERIAL AND METHODS: We prepared a nanoemulsion carrying both iodine and docetaxel in a single structure by optimizing the composition of surfactants surrounding the inner iodized oil core. CT was performed 12 hours after subcutaneous injection of the emulsion in healthy rabbits (n=3) and VX2 tumor-implanted rabbits (n=6) for SLN imaging. Next, we tested the effect of treatment by histopathologically assessing the popliteal LN metastasis rate in VX2 tumor-implanted rabbits 7 days after subcutaneous injection of the emulsion (treatment group, n=17) and comparing it with that of non-treatment group rabbits (n=17). CONCLUSIONS: We developed an iodine-docetaxel emulsion and demonstrated that it can be applied to simultaneously achieve CT SLN imaging and local chemotherapy against nodal metastasis.
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1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
김상겸(Kim, Sang Kyum) ORCID logo https://orcid.org/0000-0003-0768-9923
김한솔(Kim, Hon Soul)
임준석(Lim, Joon Seok) ORCID logo https://orcid.org/0000-0002-0334-5042
형우진(Hyung, Woo Jin) ORCID logo https://orcid.org/0000-0002-8593-9214
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