0 268

Cited 13 times in

In vivo bone formation by human alveolar-bone-derived mesenchymal stem cells obtained during implant osteotomy using biphasic calcium phosphate ceramics or Bio-Oss as carriers

Authors
 Jung-Chul Park  ;  Sang-Yeob Oh  ;  Jung-Seok Lee  ;  So-Yon Park  ;  Eun-Young Choi  ;  Kyoo-Sung Cho  ;  Chang-Sung Kim 
Citation
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, Vol.104(3) : 515-524, 2016 
Journal Title
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 
ISSN
 1552-4973 
Issue Date
2016
MeSH
Bone Matrix/cytology ; Bone Matrix/metabolism* ; Cells, Cultured ; Ceramics*/chemistry ; Ceramics*/pharmacology ; Humans ; Hydroxyapatites*/chemistry ; Hydroxyapatites*/pharmacology ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/metabolism* ; Minerals*/chemistry ; Minerals*/pharmacology ; Osteogenesis/drug effects*
Keywords
biphasic calcium phosphate ; mesenchymal stem cell ; osteogenic differentiation ; regeneration
Abstract
OBJECTIVES: The aim of this study was to evaluate HA coated with different ratios of TCP as a carrier for hABMSCs obtained during implant osteotomy in comparison to slowly-resorbing biomaterial, Bio-Oss, as a negative control, using in vitro and in vivo experiments. MATERIALS AND METHODS: Human ABMSCs (hABMSCs) harvested during implant osteotomy were transplanted using HA/TCP or Bio-Oss as carriers in a murine ectopic transplantation model (n = 12). Pore size and cell affinity were evaluated in vitro. The area of newly formed bone was analyzed histometrically, the number of osteocytes was counted, and immunohistochemical staining was conducted against several markers of osteogenesis, including alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX-2), osteocalcin (OCN), and osteopontin (OPN). Osteoclast formation was evaluated by tartrate-resistant acid phosphatase staining. RESULTS: The carrier materials had comparable pore sizes. The cell affinity assay resulted in a high proportion of cell adhesion (>90%) in all experimental groups. Substantial new bone and osteocyte formation was observed on both HA/TCP carriers, whereas it was minimal with Bio-Oss. Positive immunostaining for ALP, RUNX-2, OCN, and OPN was observed with HA/TCP, but only limited expression of osteogenic markers with Bio-Oss. Conversely, there was a minimal osteoclast presence with Bio-Oss, but a significant presence of osteoclasts with both HA/TCP carriers. CONCLUSIONS: Both types of scaffolds, BCP and Bio-Oss, showed high stem cell-carrying potential, but the in vivo healing patterns of their complexes with hABMSC could be affected by the microenvironment on the surfaces of the scaffolds.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33416/abstract
DOI
10.1002/jbm.b.33416
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152569
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links