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Troglitazone Enhances the Apoptotic Response of DLD-1 Colon Cancer Cells to Photodynamic Therapy

Authors
 Hyunju Park  ;  Si-Hwan Ko  ;  Jae Myun Lee  ;  Jeon Han Park  ;  Youn-Hee Choi 
Citation
 YONSEI MEDICAL JOURNAL, Vol.57(6) : 1494-1499, 2016 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
2016
MeSH
Antineoplastic Agents/pharmacology ; Antineoplastic Agents/therapeutic use ; Apoptosis/drug effects* ; Blotting, Western ; Caspase 3 ; Cell Cycle/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects* ; Cell Survival/drug effects* ; Chromans/pharmacology* ; Colonic Neoplasms/metabolism ; Colonic Neoplasms/pathology ; Dose-Response Relationship, Drug ; Humans ; PPAR gamma/metabolism ; PPAR gamma/pharmacology* ; Photochemotherapy* ; Tetrazolium Salts ; Thiazoles ; Thiazolidinediones/pharmacology* ; Thiazolidinediones/therapeutic use
Keywords
PDT ; apoptosis ; troglitazone
Abstract
PURPOSE: The aim of this study was to investigate whether the peroxisomal proliferator-activated receptor gamma (PPARγ) ligand troglitazone in combination with photodynamic therapy (PDT) enhances the apoptotic response of DLD-1 colon cancer cells. MATERIALS AND METHODS: The effects of troglitazone, PDT, and troglitazone in combination with PDT on cell viability and apoptosis were assessed in DLD-1 cells. Cell viability and proliferation were evaluated using the tetrazolium-based MTT assay, and apoptosis was evaluated via cell staining with propidium iodide (PI) and annexin V-FITC. The levels of pro-caspase-3 were measured via Western blot analyses. RESULTS: Treatment of troglitazone and PDT induced the growth retardation and cell death of DLD-1 cells in a dose-dependent manner, respectively. The combination treatment significantly suppressed cell growth and increased the apoptotic response of DLD-1 and resulted in apoptosis rather than necrosis, as shown by PI/annexin V staining and degradation of procaspase-3. CONCLUSION: These results document the anti-proliferative and apoptotic activities of PDT in combination with the PPARγ ligand troglitazone and provide a strong rationale for testing the therapeutic potential of combination treatment in colon cancer.
Files in This Item:
T201604143.pdf Download
DOI
10.3349/ymj.2016.57.6.1494
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152308
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