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Cited 3 times in

In Situ Pluripotency Factor Expression Promotes Functional Recovery From Cerebral Ischemia

DC Field Value Language
dc.contributor.author김명선-
dc.contributor.author김형범-
dc.contributor.author유지혜-
dc.contributor.author조성래-
dc.date.accessioned2017-10-26T07:35:07Z-
dc.date.available2017-10-26T07:35:07Z-
dc.date.issued2016-
dc.identifier.issn1525-0016-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/152225-
dc.description.abstractRecovery from ischemic tissue injury can be promoted by cell proliferation and neovascularization. Transient expression of four pluripotency factors (Pou5f1, Sox2, Myc, and Klf4) has been used to convert cell types but never been tested as a means to promote functional recovery from ischemic injury. Here we aimed to determine whether transient in situ pluripotency factor expression can improve neurobehavioral function. Cerebral ischemia was induced by transient bilateral common carotid artery occlusion, after which the four pluripotency factors were expressed through either doxycycline administration into the lateral ventricle in transgenic mice in which the four factors are expressed in a doxycycline-inducible manner. Histologic evaluation showed that this transient expression induced the proliferative generation of astrocytes and/or neural progenitors, but not neurons or glial scar, and increased neovascularization with upregulation of angiogenic factors. Furthermore, in vivo pluripotency factor expression caused neuroprotective effects such as increased numbers of mature neurons and levels of synaptic markers in the striatum. Dysplasia or tumor development was not observed. Importantly, neurobehavioral evaluations such as rotarod and ladder walking tests showed that the expression of the four factors dramatically promoted functional restoration from ischemic injury. These results provide a basis for novel therapeutic modality development for cerebral ischemia.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherAcademic Press-
dc.relation.isPartOfMOLECULAR THERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAstrocytes/metabolism-
dc.subject.MESHBrain Ischemia/genetics*-
dc.subject.MESHBrain Ischemia/physiopathology*-
dc.subject.MESHCell Count-
dc.subject.MESHCell Line-
dc.subject.MESHCorpus Striatum/metabolism-
dc.subject.MESHCorpus Striatum/pathology-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHGene Expression*-
dc.subject.MESHGenes, myc-
dc.subject.MESHKruppel-Like Transcription Factors/genetics-
dc.subject.MESHLateral Ventricles/metabolism-
dc.subject.MESHLateral Ventricles/pathology-
dc.subject.MESHMice-
dc.subject.MESHMice, Transgenic-
dc.subject.MESHNeovascularization, Pathologic/genetics-
dc.subject.MESHNeural Stem Cells/metabolism-
dc.subject.MESHOctamer Transcription Factor-3/genetics-
dc.subject.MESHRecovery of Function/genetics*-
dc.subject.MESHSOXB1 Transcription Factors/genetics-
dc.titleIn Situ Pluripotency Factor Expression Promotes Functional Recovery From Cerebral Ischemia-
dc.typeArticle-
dc.publisher.locationUnited States-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentYonsei Biomedical Research Center-
dc.contributor.googleauthorJung Hwa Seo-
dc.contributor.googleauthorMin-Young Lee-
dc.contributor.googleauthorJi Hea Yu-
dc.contributor.googleauthorMyung-Sun Kim-
dc.contributor.googleauthorMyungjae Song-
dc.contributor.googleauthorCheong Hoon Seo-
dc.contributor.googleauthorHyongbum (Henry) Kim-
dc.contributor.googleauthorSung-Rae Cho-
dc.identifier.doi10.1038/mt.2016.124-
dc.contributor.localIdA01148-
dc.contributor.localIdA02521-
dc.contributor.localIdA03831-
dc.contributor.localIdA00422-
dc.relation.journalcodeJ02271-
dc.identifier.eissn1525-0024-
dc.identifier.pmid27455881-
dc.contributor.alternativeNameKim, Myung Sun-
dc.contributor.alternativeNameKim, Hyongbum-
dc.contributor.alternativeNameYu, Ji Hea-
dc.contributor.alternativeNameCho, Sung Rae-
dc.contributor.affiliatedAuthorKim, Hyongbum-
dc.contributor.affiliatedAuthorYu, Ji Hea-
dc.contributor.affiliatedAuthorCho, Sung Rae-
dc.contributor.affiliatedAuthorKim, Myung Sun-
dc.citation.volume24-
dc.citation.number9-
dc.citation.startPage1538-
dc.citation.endPage1549-
dc.identifier.bibliographicCitationMOLECULAR THERAPY, Vol.24(9) : 1538-1549, 2016-
dc.date.modified2017-10-24-
dc.identifier.rimsid47001-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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