Cited 30 times in
Astroglial Activation by an Enriched Environment after Transplantation of Mesenchymal Stem Cells Enhances Angiogenesis after Hypoxic-Ischemic Brain Injury
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김형범 | - |
dc.contributor.author | 유지혜 | - |
dc.contributor.author | 조성래 | - |
dc.date.accessioned | 2017-10-26T07:24:25Z | - |
dc.date.available | 2017-10-26T07:24:25Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151989 | - |
dc.description.abstract | Transplantation of mesenchymal stem cells (MSCs) has paracrine effects; however, the effects are known to be largely limited. Here we investigated the combination effects of cell transplantation and enriched environment (EE) in a model of hypoxic-ischemic brain injury. Brain damage was induced in seven-day-old mice by unilateral carotid artery ligation and exposure to hypoxia (8% O₂ for 90 min). At six weeks of age, the mice were randomly assigned to four groups: phosphate-buffered saline (PBS)-control (CON), PBS-EE, MSC-CON, and MSC-EE. Rotarod and grip strength tests were performed to evaluate neurobehavioral functions. Histologic evaluations were also performed to confirm the extent of astrocyte activation and endogenous angiogenesis. An array-based multiplex ELISA and Western blot were used to identify growth factors in vivo and in vitro. Two weeks after treatment, levels of astrocyte density and angiogenic factors were increased in MSC-EE mice, but glial scarring was not increased. Eight weeks after treatment, angiogenesis was increased, and behavioral outcomes were synergistically improved in the MSC-EE group. Astrocytes co-cultured with MSCs expressed higher levels of angiogenic factors than astrocytes cultured alone. The mechanisms of this synergistic effect included enhanced repair processes, such as increased endogenous angiogenesis and upregulation of angiogenic factors released from activated astrocytes. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.publisher | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Astrocytes/cytology | - |
dc.subject.MESH | Astrocytes/metabolism | - |
dc.subject.MESH | Astrocytes/physiology* | - |
dc.subject.MESH | Blood Vessels/physiopathology* | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Brain Injuries/etiology | - |
dc.subject.MESH | Brain Injuries/physiopathology | - |
dc.subject.MESH | Brain Injuries/therapy* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Hypoxia-Ischemia, Brain/complications | - |
dc.subject.MESH | Hypoxia-Ischemia, Brain/physiopathology | - |
dc.subject.MESH | Intercellular Signaling Peptides and Proteins/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mesenchymal Stem Cell Transplantation/methods* | - |
dc.subject.MESH | Mesenchymal Stromal Cells/cytology | - |
dc.subject.MESH | Mesenchymal Stromal Cells/physiology* | - |
dc.subject.MESH | Mice, Inbred ICR | - |
dc.subject.MESH | Microscopy, Confocal | - |
dc.subject.MESH | Motor Activity/physiology | - |
dc.subject.MESH | Neuroglia/cytology | - |
dc.subject.MESH | Neuroglia/metabolism | - |
dc.subject.MESH | Neuroglia/physiology | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/metabolism | - |
dc.title | Astroglial Activation by an Enriched Environment after Transplantation of Mesenchymal Stem Cells Enhances Angiogenesis after Hypoxic-Ischemic Brain Injury | - |
dc.type | Article | - |
dc.publisher.location | Switzerland | - |
dc.contributor.college | College of Medicine | - |
dc.contributor.department | Dept. of Pharmacology | - |
dc.contributor.googleauthor | Sung-Rae Cho | - |
dc.contributor.googleauthor | Hwal Suh | - |
dc.contributor.googleauthor | Ji Hea Yu | - |
dc.contributor.googleauthor | Hyongbum (Henry) Kim | - |
dc.contributor.googleauthor | Jung Hwa Seo | - |
dc.contributor.googleauthor | Cheong Hoon Seo | - |
dc.identifier.doi | 10.3390/ijms17091550 | - |
dc.contributor.localId | A02521 | - |
dc.contributor.localId | A03831 | - |
dc.contributor.localId | A01148 | - |
dc.relation.journalcode | J01133 | - |
dc.identifier.eissn | 1422-0067 | - |
dc.identifier.pmid | 27649153 | - |
dc.subject.keyword | angiogenesis | - |
dc.subject.keyword | astrocytes | - |
dc.subject.keyword | enriched environment | - |
dc.subject.keyword | hypoxic-ischemic brain injury | - |
dc.subject.keyword | mesenchymal stem cells | - |
dc.contributor.alternativeName | Kim, Hyongbum | - |
dc.contributor.alternativeName | Yu, Ji Hea | - |
dc.contributor.alternativeName | Cho, Sung Rae | - |
dc.contributor.affiliatedAuthor | Yu, Ji Hea | - |
dc.contributor.affiliatedAuthor | Cho, Sung Rae | - |
dc.contributor.affiliatedAuthor | Kim, Hyongbum | - |
dc.citation.volume | 17 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 1550 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.17(9) : 1550, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 46311 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.