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Astroglial Activation by an Enriched Environment after Transplantation of Mesenchymal Stem Cells Enhances Angiogenesis after Hypoxic-Ischemic Brain Injury

DC Field Value Language
dc.contributor.author김형범-
dc.contributor.author유지혜-
dc.contributor.author조성래-
dc.date.accessioned2017-10-26T07:24:25Z-
dc.date.available2017-10-26T07:24:25Z-
dc.date.issued2016-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/151989-
dc.description.abstractTransplantation of mesenchymal stem cells (MSCs) has paracrine effects; however, the effects are known to be largely limited. Here we investigated the combination effects of cell transplantation and enriched environment (EE) in a model of hypoxic-ischemic brain injury. Brain damage was induced in seven-day-old mice by unilateral carotid artery ligation and exposure to hypoxia (8% O₂ for 90 min). At six weeks of age, the mice were randomly assigned to four groups: phosphate-buffered saline (PBS)-control (CON), PBS-EE, MSC-CON, and MSC-EE. Rotarod and grip strength tests were performed to evaluate neurobehavioral functions. Histologic evaluations were also performed to confirm the extent of astrocyte activation and endogenous angiogenesis. An array-based multiplex ELISA and Western blot were used to identify growth factors in vivo and in vitro. Two weeks after treatment, levels of astrocyte density and angiogenic factors were increased in MSC-EE mice, but glial scarring was not increased. Eight weeks after treatment, angiogenesis was increased, and behavioral outcomes were synergistically improved in the MSC-EE group. Astrocytes co-cultured with MSCs expressed higher levels of angiogenic factors than astrocytes cultured alone. The mechanisms of this synergistic effect included enhanced repair processes, such as increased endogenous angiogenesis and upregulation of angiogenic factors released from activated astrocytes.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherMDPI-
dc.relation.isPartOfINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAstrocytes/cytology-
dc.subject.MESHAstrocytes/metabolism-
dc.subject.MESHAstrocytes/physiology*-
dc.subject.MESHBlood Vessels/physiopathology*-
dc.subject.MESHBlotting, Western-
dc.subject.MESHBrain Injuries/etiology-
dc.subject.MESHBrain Injuries/physiopathology-
dc.subject.MESHBrain Injuries/therapy*-
dc.subject.MESHCells, Cultured-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHFemale-
dc.subject.MESHHypoxia-Ischemia, Brain/complications-
dc.subject.MESHHypoxia-Ischemia, Brain/physiopathology-
dc.subject.MESHIntercellular Signaling Peptides and Proteins/metabolism-
dc.subject.MESHMale-
dc.subject.MESHMesenchymal Stem Cell Transplantation/methods*-
dc.subject.MESHMesenchymal Stromal Cells/cytology-
dc.subject.MESHMesenchymal Stromal Cells/physiology*-
dc.subject.MESHMice, Inbred ICR-
dc.subject.MESHMicroscopy, Confocal-
dc.subject.MESHMotor Activity/physiology-
dc.subject.MESHNeuroglia/cytology-
dc.subject.MESHNeuroglia/metabolism-
dc.subject.MESHNeuroglia/physiology-
dc.subject.MESHTime Factors-
dc.subject.MESHVascular Endothelial Growth Factor A/metabolism-
dc.titleAstroglial Activation by an Enriched Environment after Transplantation of Mesenchymal Stem Cells Enhances Angiogenesis after Hypoxic-Ischemic Brain Injury-
dc.typeArticle-
dc.publisher.locationSwitzerland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Pharmacology-
dc.contributor.googleauthorSung-Rae Cho-
dc.contributor.googleauthorHwal Suh-
dc.contributor.googleauthorJi Hea Yu-
dc.contributor.googleauthorHyongbum (Henry) Kim-
dc.contributor.googleauthorJung Hwa Seo-
dc.contributor.googleauthorCheong Hoon Seo-
dc.identifier.doi10.3390/ijms17091550-
dc.contributor.localIdA02521-
dc.contributor.localIdA03831-
dc.contributor.localIdA01148-
dc.relation.journalcodeJ01133-
dc.identifier.eissn1422-0067-
dc.identifier.pmid27649153-
dc.subject.keywordangiogenesis-
dc.subject.keywordastrocytes-
dc.subject.keywordenriched environment-
dc.subject.keywordhypoxic-ischemic brain injury-
dc.subject.keywordmesenchymal stem cells-
dc.contributor.alternativeNameKim, Hyongbum-
dc.contributor.alternativeNameYu, Ji Hea-
dc.contributor.alternativeNameCho, Sung Rae-
dc.contributor.affiliatedAuthorYu, Ji Hea-
dc.contributor.affiliatedAuthorCho, Sung Rae-
dc.contributor.affiliatedAuthorKim, Hyongbum-
dc.citation.volume17-
dc.citation.number9-
dc.citation.startPage1550-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.17(9) : 1550, 2016-
dc.date.modified2017-10-24-
dc.identifier.rimsid46311-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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