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Different Responses in Induction of Allergen Specific Immunoglobulin G4 and IgE-Blocking Factors for Three Mite Subcutaneous Immunotherapy Products

 Kyung Hee Park  ;  Sang Chul Lee  ;  Young Woong Son  ;  Kyoung Yong Jeong  ;  Yoo Seob Shin  ;  Jung U Shin  ;  Da Woon Sim  ;  Hye Jung Park  ;  Jae-Hyun Lee  ;  Kwang Hoon Lee  ;  Jung-Won Park 
 YONSEI MEDICAL JOURNAL, Vol.57(6) : 1427-1434, 2016 
Journal Title
Issue Date
Adult ; Allergens/administration & dosage ; Allergens/immunology* ; Animals ; Antibody Specificity/immunology ; Antigens, Dermatophagoides ; Antigens, Neoplasm* ; Dermatitis, Atopic/blood ; Dermatitis, Atopic/immunology* ; Dermatitis, Atopic/therapy* ; Desensitization, Immunologic/methods* ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin E/blood ; Immunoglobulin E/immunology* ; Immunoglobulin G/blood ; Immunoglobulin G/immunology* ; Injections, Subcutaneous ; Male ; Mites/immunology* ; Pyroglyphidae/immunology ; Treatment Outcome ; Young Adult
Blocking factor ; IgG4 ; house dust mite ; immunotherapy ; specific IgE
PURPOSE: Specific immunoglobulin G4 (sIgG4) and immunoglobulin E (IgE)-blocking factors produced by subcutaneous immunotherapy (SCIT) play a critical role in the induction of allergen tolerance. However, comparative studies of available SCIT reagents on the induction of sIgG4 are limited. We compared increases in sIgG4 for three different house dust mite (HDM) SCIT reagents.

MATERIALS AND METHODS: Seventy-two HDM sensitized allergic patients were enrolled and classified into four groups: 1) control (n=27), 2) SCIT with Hollister-Stier® (n=19), 3) Tyrosine S® (n=16), and 4) Novo-Helisen® (n=10). Levels of specific IgE (sIgE), sIgG4, and IgE blocking factor to Dermatophagoides farinae (D. farinae) were measured using ImmunoCAP (sIgE, sIgG4) and enzyme-linked immunosorbent assay (ELISA) (IgE-blocking factors). Levels were measured before and 13.9±6.6 months after the SCIT. The allergen specificity and the induction levels of sIgE and sIgG4 were confirmed by immunoblot analysis.

RESULTS: After SCIT, sIgG4 levels to D. farinae increased significantly; however, the increases differed significantly among the SCIT groups (p<0.001). Specific IgG4 levels to D. farinae were highest in Hollister-Stier® (3.7±4.1 mg/L), followed by Novo-Helisen® (2.2±2.3 mg/L) and Tyrosine S® (0.7±0.5 mg/L). In addition, patients who were administered using Hollister-Stier® showed the most significant decrease in IgE/IgG4 ratio (p<0.001) and increase in blocking factor (p=0.009). Finally, according to IgE immunoblot results, the Hollister-Stier® group showed the most significant attenuation of IgE binding patterns among others.

CONCLUSION: Currently available SCIT reagents induce different levels of specific IgG4, IgE/IgG4 ratio, and IgE-blocking factor.
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Kyung Hee(박경희) ORCID logo https://orcid.org/0000-0003-3605-5364
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Park, Hye Jung(박혜정) ORCID logo https://orcid.org/0000-0002-1862-1003
Son, Young Woong(손영웅)
Shin, Jung U(신정우) ORCID logo https://orcid.org/0000-0001-5259-6879
Sim, Da Woon(심다운)
Lee, Kwang Hoon(이광훈)
Lee, Sang Chul(이상철)
Lee, Jae Hyun(이재현) ORCID logo https://orcid.org/0000-0002-0760-0071
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
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