0 579

Cited 15 times in

Antiobesity and Antidiabetes Effects of a Cudrania tricuspidata Hydrophilic Extract Presenting PTP1B Inhibitory Potential

 Dae Hoon Kim  ;  Sooung Lee  ;  Youn Wook Chung  ;  Byeong Mo Kim  ;  Hanseul Kim  ;  Kunhong Kim  ;  Kyung Mi Yang 
 BIOMED RESEARCH INTERNATIONAL, Vol.2016 : 8432759, 2016 
Journal Title
Issue Date
3T3-L1 Cells ; Animals ; Diabetes Mellitus/drug therapy* ; Diabetes Mellitus/genetics ; Diabetes Mellitus/pathology ; Humans ; Insulin/blood ; Insulin Resistance/genetics ; Liver/drug effects ; Liver/pathology ; Mice ; Mice, Obese ; Moraceae/chemistry ; Obesity/drug therapy* ; Obesity/genetics ; Obesity/pathology ; Plant Extracts/administration & dosage* ; Plant Extracts/chemistry ; Protein Tyrosine Phosphatase, Non-Receptor Type 1/biosynthesis* ; Signal Transduction/drug effects
Diabetes and obesity represent the major health problems and the most age-related metabolic diseases. Protein-tyrosine phosphatase 1B (PTP1B) has emerged as an important regulator of insulin signal transduction and is regarded as a pharmaceutical target for metabolic disorders. To find novel natural materials presenting therapeutic activities against diabetes and obesity, we screened various herb extracts using a chip screening allowing the determination of PTP1B inhibitory effects of the tested compounds using insulin receptor (IR) as the substrate. Cudrania tricuspidata leaves (CTe) had a strong inhibitory effect on PTP1B activity and substantially inhibited fat accumulation in 3T3-L1 cells. CTe was orally administrated to diet-induced obesity (DIO) mice once daily for 3 weeks after which changes in glucose, insulin metabolism, and fat accumulation were examined. Hepatic enzyme markers (aspartate aminotransferase, AST, and alanine aminotransferase, ALT) and total fat mass and triglyceride levels decreased in CTe-treated mice, whereas body weight and total cholesterol concentration slightly decreased. CTe increased the phosphorylation of IRS-1 and Akt in liver tissue. Furthermore, CTe treatment significantly lowered blood glucose levels and improved insulin secretion in DIO mice. Our results strongly suggest that CTe may represent a promising therapeutic substance against diabetes and obesity.
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kun Hong(김건홍) ORCID logo https://orcid.org/0000-0001-5639-6372
Kim, Byeong Mo(김병모) ORCID logo https://orcid.org/0000-0002-0582-3132
Yang, Kyung Mi(양경미)
Chung, Youn Wook(정연욱) ORCID logo https://orcid.org/0000-0002-4382-1410
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.