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Kelvin probe force microscopy of DNA-capped nanoparticles for single-nucleotide polymorphism detection

Authors
 Hyungbeen Lee  ;  Sang Won Lee  ;  Gyudo Lee  ;  Wonseok Lee  ;  Jeong Hoon Lee  ;  Kyo Seon Hwang  ;  Jaemoon Yang  ;  Sang Woo Lee  ;  Dae Sung Yoon 
Citation
 NANOSCALE, Vol.8(28) : 13537-13544, 2016 
Journal Title
 NANOSCALE 
ISSN
 2040-3364 
Issue Date
2016
Abstract
Kelvin probe force microscopy (KPFM) is a robust toolkit for profiling the surface potential (SP) of biomolecular interactions between DNAs and/or proteins at the single molecule level. However, it has often suffered from background noise and low throughput due to instrumental or environmental constraints, which is regarded as limiting KPFM applications for detection of minute changes in the molecular structures such as single-nucleotide polymorphism (SNP). Here, we show KPFM imaging of DNA-capped nanoparticles (DCNP) that enables SNP detection of the BRCA1 gene owing to sterically well-adjusted DNA-DNA interactions that take place within the confined spaces of DCNP. The average SP values of DCNP interacting with BRCA1 SNP were found to be lower than the DCNP reacting with normal (non-mutant) BRCA1 gene. We also demonstrate that SP characteristics of DCNP with different substrates (e.g., Au, Si, SiO2, and Fe) provide us with a chance to attenuate or augment the SP signal of DCNP without additional enhancement of instrumentation capabilities.
Full Text
http://pubs.rsc.org/en/Content/ArticleLanding/2016/NR/C5NR08969G#!divAbstract
DOI
10.1039/c5nr08969g
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151664
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