0 817

Cited 311 times in

Dynamic Axonal Translation in Developing and Mature Visual Circuits

Authors
 Toshiaki Shigeoka  ;  Hosung Jung  ;  Jane Jung  ;  Benita Turner-Bridger  ;  Jiyeon Ohk  ;  Julie Qiaojin Lin  ;  Paul S. Amieux  ;  Christine E. Holt 
Citation
 CELL, Vol.166(1) : 181-192, 2016 
Journal Title
CELL
ISSN
 0092-8674 
Issue Date
2016
MeSH
Alternative Splicing ; Animals ; Axons/metabolism* ; Gene Expression Regulation, Developmental ; Mice ; Protein Biosynthesis* ; Proteome/analysis ; Proteome/metabolism* ; RNA, Messenger/analysis ; RNA, Messenger/metabolism ; Retinal Ganglion Cells/metabolism* ; Ribosomes/metabolism ; Superior Colliculi/metabolism ; Synaptic Transmission
Abstract
Local mRNA translation mediates the adaptive responses of axons to extrinsic signals, but direct evidence that it occurs in mammalian CNS axons in vivo is scant. We developed an axon-TRAP-RiboTag approach in mouse that allows deep-sequencing analysis of ribosome-bound mRNAs in the retinal ganglion cell axons of the developing and adult retinotectal projection in vivo. The embryonic-to-postnatal axonal translatome comprises an evolving subset of enriched genes with axon-specific roles, suggesting distinct steps in axon wiring, such as elongation, pruning, and synaptogenesis. Adult axons, remarkably, have a complex translatome with strong links to axon survival, neurotransmission, and neurodegenerative disease. Translationally co-regulated mRNA subsets share common upstream regulators, and sequence elements generated by alternative splicing promote axonal mRNA translation. Our results indicate that intricate regulation of compartment-specific mRNA translation in mammalian CNS axons supports the formation and maintenance of neural circuits in vivo.
Full Text
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4930487/?report=printable
DOI
10.1016/j.cell.2016.05.029
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Jung, Ho Sung(정호성) ORCID logo https://orcid.org/0000-0002-5059-8050
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151652
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links