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침윤성 자궁경부암에서 실시간 정량 역전사 중합효소 연쇄 반응을 이용한 cyclin B1과 D1의 발현

Other Titles
 Cyclin B1 and D1 expression in invasive cervical cancer 
 김영태  ;  자오민  ;  김희연  ;  강명화  ;  김재욱  ;  김성훈  ;  김재훈  ;  김상운  ;  윤보성 
 Korean Journal of Gynecologic Oncology (부인종양), Vol.16(3) : 256-263, 2005 
Journal Title
Korean Journal of Gynecologic Oncology(부인종양)
Issue Date
Blotting, Western ; Cervix Uteri/physiology ; Cyclin B/genetics* ; Cyclin B/metabolism ; Cyclin B1 ; Cyclin D1/genetics* ; Cyclin D1/metabolism ; DNA, Complementary/genetics ; Female ; Gene Expression Profiling* ; Humans ; Neoplasm Invasiveness ; Neoplasm Staging ; RNA, Neoplasm/genetics ; Reference Values ; Reverse Transcriptase Polymerase Chain Reaction ; Uterine Cervical Neoplasms/genetics* ; Uterine Cervical Neoplasms/pathology
cervical cancer ; cyclin B1 ; cyclin D1 ; real-time RT-PCR ; Western blot
Objective : Cyclin is a family of regulatory proteins that play a key role in controlling the cell cycle. Abnormalities of cell cycle regulators, including cyclins and cyclin dependent kinases (CDKs), have been reported in malignant tumors. This study was undertaken to quantitatively detect cyclin B1 and D1 in cervical cancer.

Methods : A quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis were used to analyze the expression of cyclin B1, D1 mRNA and proteins, respectively, in fresh invasive cervical cancer (n=41) and normal cervix tissue (n=10).

Results : There was significantly greater cyclin B1 expression in invasive cervical cancer than in normal cervix tissue (p=0.019). However, cyclin D1 expression was not significantly different (p=0.967). A Western blot analysis yielded similar results.

Conclusion : Our results were consistent with the concept that up-regulation of cyclin B1 expression occurred in cervical cancer and an aberrant expression of cyclin B1 might play an important role in cervical carcinogenesis.
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kang, Myung Hwa(강명화)
Kim, Sang Wun(김상운) ORCID logo https://orcid.org/0000-0002-8342-8701
Kim, Sung Hoon(김성훈) ORCID logo https://orcid.org/0000-0002-1645-7473
Kim, Young Tae(김영태) ORCID logo https://orcid.org/0000-0002-7347-1052
Kim, Jae Wook(김재욱)
Kim, Jae Hoon(김재훈) ORCID logo https://orcid.org/0000-0001-6599-7065
Yoon, Bo Sung(윤보성)
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