160 316

Cited 0 times in

ER Stress-Induced Jpk Expression and the Concomitant Cell Death

 Hye Sun Kim  ;  Hyunjoo Chung  ;  Kyoung-Ah Kong  ;  Sungdo Park  ;  Myoung Hee Kim 
 Journal of Experimental & Biomedcal Science (대한의생명과학회지), Vol.11(2) : 135-141, 2005 
Journal Title
Journal of Experimental & Biomedcal Science(대한의생명과학회지)
Issue Date
Jpk ; Endoplasmic reticulum (ER)-stress ; Cell death
Jpk ; Endoplasmic reticulum (ER)-stress ; Cell death
A Jopock (Jpk), a trans-acting factor associating with the position-specific regulatory element of murine Hoxa-7, has shown to have a toxicity to both prokaryotic and eukaryotic cells when overexpressed. Since Jpk protein harbors a transmembrane domain and a putative endoplasmic reticulum (ER)-retention signal at the N-terminus, a subcellular localization of the protein was analyzed after fusing it into the green fluorescent protein (GFP): Both N-term (Jpk-EGFP) and C-term tagged-Jpk (EGFP-Jpk) showed to be localized in the ER when analyzed under the fluorescence microscopy after staining the cells with ER- and MitoTracker. Since ER stress triggers the ER-stress mediated apoptosis to eliminate the damaged cells, we analyzed the expression pattern of Jpk under ER-stress condition. When MCF7 cells were treated with the ER-stress inducer such as DTT and EGTA, the expression of Jpk was upregulated at the transcriptional level like that of Grp78, a molecular chaperone well known to be overexpressed under ER-stress condition. In the presence of high concentration of ER-stress inducer (10 mM), about 70 (DTT) to 95% (EGTA) of cells died stronly expressing (10~12 fold) Jpk. Whereas at the low concentration (0.001~1.0 mM) of the inducer, the expression of Jpk was increased about 2.5 (EGTA) to 5 fold (DTT), which is rather similar to those of ER chaperone protein Grp78. These results altogether indicate that the ER-stress upregulated the expression of Jpk and the excess stress induces the ER-stress induced apoptosis and the concomitant expression of Jpk.
Files in This Item:
T200501086.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kong, Kyoung Ah(공경아)
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Chung, Huyun Joo(정현주)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.