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ER Stress-Induced Jpk Expression and the Concomitant Cell Death
DC Field | Value | Language |
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dc.contributor.author | 공경아 | - |
dc.contributor.author | 김명희 | - |
dc.contributor.author | 정현주 | - |
dc.date.accessioned | 2017-10-26T06:42:17Z | - |
dc.date.available | 2017-10-26T06:42:17Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1738-3226 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151196 | - |
dc.description.abstract | A Jopock (Jpk), a trans-acting factor associating with the position-specific regulatory element of murine Hoxa-7, has shown to have a toxicity to both prokaryotic and eukaryotic cells when overexpressed. Since Jpk protein harbors a transmembrane domain and a putative endoplasmic reticulum (ER)-retention signal at the N-terminus, a subcellular localization of the protein was analyzed after fusing it into the green fluorescent protein (GFP): Both N-term (Jpk-EGFP) and C-term tagged-Jpk (EGFP-Jpk) showed to be localized in the ER when analyzed under the fluorescence microscopy after staining the cells with ER- and MitoTracker. Since ER stress triggers the ER-stress mediated apoptosis to eliminate the damaged cells, we analyzed the expression pattern of Jpk under ER-stress condition. When MCF7 cells were treated with the ER-stress inducer such as DTT and EGTA, the expression of Jpk was upregulated at the transcriptional level like that of Grp78, a molecular chaperone well known to be overexpressed under ER-stress condition. In the presence of high concentration of ER-stress inducer (10 mM), about 70 (DTT) to 95% (EGTA) of cells died stronly expressing (10~12 fold) Jpk. Whereas at the low concentration (0.001~1.0 mM) of the inducer, the expression of Jpk was increased about 2.5 (EGTA) to 5 fold (DTT), which is rather similar to those of ER chaperone protein Grp78. These results altogether indicate that the ER-stress upregulated the expression of Jpk and the excess stress induces the ER-stress induced apoptosis and the concomitant expression of Jpk. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | Korean, English | - |
dc.publisher | Korean Society For Biomedical Laboratory Sciences | - |
dc.relation.isPartOf | Journal of Experimental & Biomedcal Science (대한의생명과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Jpk | - |
dc.subject.MESH | Endoplasmic reticulum (ER)-stress | - |
dc.subject.MESH | Cell death | - |
dc.title | ER Stress-Induced Jpk Expression and the Concomitant Cell Death | - |
dc.type | Article | - |
dc.publisher.location | Korea | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학교실) | - |
dc.contributor.department | Dept. of Anatomy (해부학교실) | - |
dc.contributor.department | Dept. of Anatomy (해부학교실) | - |
dc.contributor.googleauthor | Hye Sun Kim | - |
dc.contributor.googleauthor | Hyunjoo Chung | - |
dc.contributor.googleauthor | Kyoung-Ah Kong | - |
dc.contributor.googleauthor | Sungdo Park | - |
dc.contributor.googleauthor | Myoung Hee Kim | - |
dc.identifier.doi | OAK-2005-05372 | - |
dc.contributor.localId | A00158 | - |
dc.contributor.localId | A00432 | - |
dc.contributor.localId | A03769 | - |
dc.relation.journalcode | J01408 | - |
dc.relation.journalsince | 2004~ | - |
dc.relation.journalbefore | ~2004 Journal of Biomedical Laboratory Sciences (대한의생명과학회지) | - |
dc.subject.keyword | Jpk | - |
dc.subject.keyword | Endoplasmic reticulum (ER)-stress | - |
dc.subject.keyword | Cell death | - |
dc.contributor.alternativeName | Kong, Kyoung Ah | - |
dc.contributor.alternativeName | Kim, Myoung Hee | - |
dc.contributor.alternativeName | Chung, Huyun Joo | - |
dc.citation.volume | 11 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 135 | - |
dc.citation.endPage | 141 | - |
dc.identifier.bibliographicCitation | Journal of Experimental & Biomedcal Science (대한의생명과학회지), Vol.11(2) : 135-141, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 43528 | - |
dc.type.rims | ART | - |
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