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Synergistic Induction of Cyclooxygenase-2/Inducible Nitric Oxide Synthase Gene by Bacterial Lipopolysaccharide in Combinationwith Silica Parti-cles in RAW 264.7 Macrophages

 Dong Hee Lee  ;  Bong Joo Park  ;  Maki Aihara  ;  Jong-Chul Park  ;  Kosuke Takatori 
 Biomaterials Research (생체재료학회지), Vol.9(3) : 145-149, 2005 
Journal Title
Biomaterials Research(생체재료학회지)
Issue Date
Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are useful tool for the identification of inflammatory potentials of materials for medical device more specifically than present in vivo assays. The aim of the present study was to investigate the effects of silica particles on the expression of COX-2/iNOS mRNA gene in cultured murine macrophage cells. Silica particles significantly induced COX-2 and iNOS mRNA expression in RAW 264.7 macrophages in a dose-dependent manner. Additionally, in this study we demonstrated synergistic induction of the COX-2/iNOS mRNA expression by the combination of the lipopolysaccharide (LPS) with silica particles in RAW 264.7 cells. When the silica particles were added together with a minute amount of LPS, the levels of the COX-2 and iNOS mRNA expressions were increased several-fold greater than the addition of any inducer alone. This demonstrates that a concentration of LPS, such as would be found associated with a bacterial infection at the site of implanted biomaterials, induces a significant production of inflammatory mediators by macrophages.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Lee, Dong Hee(이동희)
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