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Murine Model of Buckwheat Allergy by Intragastric Sensitization with Fresh Buckwheat Flour Extract

Authors
 Soo-Young Lee  ;  Sejo Oh  ;  Kisun Lee  ;  Young-Ju Jang  ;  Myung-Hyun Sohn  ;  Kyoung-En Lee  ;  Kyu-Earn Kim 
Citation
 JOURNAL OF KOREAN MEDICAL SCIENCE, Vol.20(4) : 566-572, 2005 
Journal Title
 JOURNAL OF KOREAN MEDICAL SCIENCE 
ISSN
 1011-8934 
Issue Date
2005
MeSH
Anaphylaxis/blood ; Anaphylaxis/immunology ; Animals ; Cell Proliferation/drug effects ; Disease Models, Animal ; Dose-Response Relationship, Drug ; Enzyme-Linked Immunosorbent Assay ; Fagopyrum/immunology* ; Female ; Flour* ; Food Hypersensitivity/blood ; Food Hypersensitivity/immunology* ; Immunoglobulin E/blood ; Immunoglobulin E/immunology ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Interferon-gamma/biosynthesis ; Interleukin-4/biosynthesis ; Interleukin-5/biosynthesis ; Mice ; Mice, Inbred C3H ; Plant Extracts/administration & dosage ; Plant Extracts/immunology ; Spleen/cytology ; Spleen/drug effects ; Spleen/metabolism ; Stomach/drug effects ; Stomach/immunology* ; T-Lymphocytes/cytology ; T-Lymphocytes/drug effects ; T-Lymphocytes/metabolism ; Time Factors
Keywords
Buckwheat ; Disease Models ; Animal ; Food Hypersensitivity
Abstract
Food allergies affect about 4% of the Korean population, and buckwheat allergy is one of the most severe food allergies in Korea. The purpose of the present study was to develop a murine model of IgE-mediated buckwheat hypersensitivity induced by intragastric sensitization. Young female C3H/HeJ mice were sensitized and challenged intragastricly with fresh buckwheat flour (1, 5, 25 mg/dose of proteins) mixed in cholera toxin, followed by intragastric challenge. Anaphylactic reactions, antigen-specific antibodies, splenocytes proliferation assays and cytokine productions were evaluated. Oral buckwheat challenges of sensitized mice provoked anaphylactic reactions such as severe scratch, perioral/periorbital swellings, or decreased activity. Reactions were associated with elevated levels of buckwheat-specific IgE antibodies. Splenocytes from buckwheat allergic mice exhibited significantly greater proliferative responses to buckwheat than non-allergic mice. Buckwheat-stimulated IL-4, IL-5, and INF-γ productions were associated with elevated levels of buckwheat-specific IgE in sensitized mice. In this model, 1 mg and 5 mg dose of sensitization produced almost the same degree of Th2-directed immune response, however, a 25 mg dose showed blunted antibody responses. In conclusion, we developed IgE-mediated buckwheat allergy by intragastric sensitization and challenge, and this model could provide a good tool for future studies.
Files in This Item:
T200500693.pdf Download
DOI
10.3346/jkms.2005.20.4.566
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Earn(김규언)
Sohn, Myung Hyun(손명현) ORCID logo https://orcid.org/0000-0002-2478-487X
Lee, Kyung Eun(이경은)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/150568
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