Cited 16 times in
Murine Model of Buckwheat Allergy by Intragastric Sensitization with Fresh Buckwheat Flour Extract
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김규언 | - |
dc.contributor.author | 손명현 | - |
dc.contributor.author | 이경은 | - |
dc.date.accessioned | 2017-10-26T06:08:49Z | - |
dc.date.available | 2017-10-26T06:08:49Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1011-8934 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/150568 | - |
dc.description.abstract | Food allergies affect about 4% of the Korean population, and buckwheat allergy is one of the most severe food allergies in Korea. The purpose of the present study was to develop a murine model of IgE-mediated buckwheat hypersensitivity induced by intragastric sensitization. Young female C3H/HeJ mice were sensitized and challenged intragastricly with fresh buckwheat flour (1, 5, 25 mg/dose of proteins) mixed in cholera toxin, followed by intragastric challenge. Anaphylactic reactions, antigen-specific antibodies, splenocytes proliferation assays and cytokine productions were evaluated. Oral buckwheat challenges of sensitized mice provoked anaphylactic reactions such as severe scratch, perioral/periorbital swellings, or decreased activity. Reactions were associated with elevated levels of buckwheat-specific IgE antibodies. Splenocytes from buckwheat allergic mice exhibited significantly greater proliferative responses to buckwheat than non-allergic mice. Buckwheat-stimulated IL-4, IL-5, and INF-γ productions were associated with elevated levels of buckwheat-specific IgE in sensitized mice. In this model, 1 mg and 5 mg dose of sensitization produced almost the same degree of Th2-directed immune response, however, a 25 mg dose showed blunted antibody responses. In conclusion, we developed IgE-mediated buckwheat allergy by intragastric sensitization and challenge, and this model could provide a good tool for future studies. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | 대한의학회(The Korean Academy of Medical Sciences) | - |
dc.relation.isPartOf | JOURNAL OF KOREAN MEDICAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anaphylaxis/blood | - |
dc.subject.MESH | Anaphylaxis/immunology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Proliferation/drug effects | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Fagopyrum/immunology* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Flour* | - |
dc.subject.MESH | Food Hypersensitivity/blood | - |
dc.subject.MESH | Food Hypersensitivity/immunology* | - |
dc.subject.MESH | Immunoglobulin E/blood | - |
dc.subject.MESH | Immunoglobulin E/immunology | - |
dc.subject.MESH | Immunoglobulin G/blood | - |
dc.subject.MESH | Immunoglobulin G/immunology | - |
dc.subject.MESH | Interferon-gamma/biosynthesis | - |
dc.subject.MESH | Interleukin-4/biosynthesis | - |
dc.subject.MESH | Interleukin-5/biosynthesis | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred C3H | - |
dc.subject.MESH | Plant Extracts/administration & dosage | - |
dc.subject.MESH | Plant Extracts/immunology | - |
dc.subject.MESH | Spleen/cytology | - |
dc.subject.MESH | Spleen/drug effects | - |
dc.subject.MESH | Spleen/metabolism | - |
dc.subject.MESH | Stomach/drug effects | - |
dc.subject.MESH | Stomach/immunology* | - |
dc.subject.MESH | T-Lymphocytes/cytology | - |
dc.subject.MESH | T-Lymphocytes/drug effects | - |
dc.subject.MESH | T-Lymphocytes/metabolism | - |
dc.subject.MESH | Time Factors | - |
dc.title | Murine Model of Buckwheat Allergy by Intragastric Sensitization with Fresh Buckwheat Flour Extract | - |
dc.type | Article | - |
dc.publisher.location | Korea (South) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pediatrics (소아청소년과학교실) | - |
dc.contributor.department | Dept. of Pediatrics (소아청소년과학교실) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Soo-Young Lee | - |
dc.contributor.googleauthor | Sejo Oh | - |
dc.contributor.googleauthor | Kisun Lee | - |
dc.contributor.googleauthor | Young-Ju Jang | - |
dc.contributor.googleauthor | Myung-Hyun Sohn | - |
dc.contributor.googleauthor | Kyoung-En Lee | - |
dc.contributor.googleauthor | Kyu-Earn Kim | - |
dc.identifier.doi | 10.3346/jkms.2005.20.4.566 | - |
dc.contributor.localId | A00327 | - |
dc.contributor.localId | A01967 | - |
dc.contributor.localId | A02651 | - |
dc.relation.journalcode | J01517 | - |
dc.identifier.eissn | 1598-6357 | - |
dc.identifier.pmid | 16100445 | - |
dc.subject.keyword | Buckwheat | - |
dc.subject.keyword | Disease Models | - |
dc.subject.keyword | Animal | - |
dc.subject.keyword | Food Hypersensitivity | - |
dc.contributor.alternativeName | Kim, Kyu Earn | - |
dc.contributor.alternativeName | Son, Myung Hyun | - |
dc.contributor.alternativeName | Lee, Kyung Eun | - |
dc.citation.volume | 20 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 566 | - |
dc.citation.endPage | 572 | - |
dc.identifier.bibliographicCitation | JOURNAL OF KOREAN MEDICAL SCIENCE, Vol.20(4) : 566-572, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 44141 | - |
dc.type.rims | ART | - |
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