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Cellular death mediated by nuclear factor kappa B (NF-κB) translocation in cultured human lens epithelial cells after ultraviolet-B irradiation

Authors
 Do Hyung Lee  ;  Kyung Sun Cho  ;  Sang-Gyu Park  ;  Eung Kwon Kim  ;  Choun-Ki Joo 
Citation
 JOURNAL OF CATARACT AND REFRACTIVE SURGERY, Vol.31(3) : 614-619, 2005 
Journal Title
 JOURNAL OF CATARACT AND REFRACTIVE SURGERY 
ISSN
 0886-3350 
Issue Date
2005
MeSH
Apoptosis* ; Cell Survival ; Cells, Cultured ; Electrophoretic Mobility Shift Assay ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Epithelial Cells/radiation effects* ; Fluorescent Antibody Technique, Indirect ; Humans ; Lens, Crystalline/cytology* ; Microscopy, Fluorescence ; NF-kappa B/antagonists & inhibitors ; NF-kappa B/metabolism* ; Sulfasalazine/pharmacology ; Ultraviolet Rays*
Keywords
15811753
Abstract
PURPOSE: To determine the role of nuclear factor kappa B (NF-kappaB) in the death of lens epithelial cells (LECs) after ultraviolet (UV) irradiation. SETTING: Department of Ophthalmology, Ilsan Paik Hospital, Inje University, Korea. METHODS: Cultures of simian virus 40 transfected human LECs (HLE B-3 cells) were were irradiated with a UVB source (312 nm) located 10 cm from the bottom of the slides for 1, 2, 3, or 4 minutes. Cytotoxicity was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Translocation of NF-kappaB was examined by immunocytochemistry using anti-NF-kappaB p65 antibody and electrophoretic mobility shift assay (EMSA). Sulfasalazine, a specific NF-kappaB inhibitor, was used to confirm the role of NF-kappaB by pretreating samples for 30 minutes before UV irradiation, after which cytotoxicity and NF-kappaB translocation were evaluated. RESULTS: When HLE B-3 cells were irradiated with UVB, translocation of NF-kappaB was observed with immunocytochemistry. These translocations peaked during EMSA 6 hours after UV irradiation. In HLE B-3 cells pretreated with sulfasalazine, the translocation of NF-kappaB was blocked. Cellular death after UV irradiation was also markedly reduced by sulfasalazine pretreatment. Ultraviolet irradiation can translocate NF-kappaB, and sulfasalazine is a useful blocking agent in this pathway. In this experimental model, sulfasalazine prevented cellular death after UV irradiation.
Full Text
http://www.sciencedirect.com/science/article/pii/S0886335004006571
DOI
10.1016/j.jcrs.2004.05.053
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147670
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