Cited 17 times in
Cellular death mediated by nuclear factor kappa B (NF-κB) translocation in cultured human lens epithelial cells after ultraviolet-B irradiation
DC Field | Value | Language |
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dc.contributor.author | 김응권 | - |
dc.date.accessioned | 2017-05-08T08:01:12Z | - |
dc.date.available | 2017-05-08T08:01:12Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0886-3350 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/147670 | - |
dc.description.abstract | PURPOSE: To determine the role of nuclear factor kappa B (NF-kappaB) in the death of lens epithelial cells (LECs) after ultraviolet (UV) irradiation. SETTING: Department of Ophthalmology, Ilsan Paik Hospital, Inje University, Korea. METHODS: Cultures of simian virus 40 transfected human LECs (HLE B-3 cells) were were irradiated with a UVB source (312 nm) located 10 cm from the bottom of the slides for 1, 2, 3, or 4 minutes. Cytotoxicity was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Translocation of NF-kappaB was examined by immunocytochemistry using anti-NF-kappaB p65 antibody and electrophoretic mobility shift assay (EMSA). Sulfasalazine, a specific NF-kappaB inhibitor, was used to confirm the role of NF-kappaB by pretreating samples for 30 minutes before UV irradiation, after which cytotoxicity and NF-kappaB translocation were evaluated. RESULTS: When HLE B-3 cells were irradiated with UVB, translocation of NF-kappaB was observed with immunocytochemistry. These translocations peaked during EMSA 6 hours after UV irradiation. In HLE B-3 cells pretreated with sulfasalazine, the translocation of NF-kappaB was blocked. Cellular death after UV irradiation was also markedly reduced by sulfasalazine pretreatment. Ultraviolet irradiation can translocate NF-kappaB, and sulfasalazine is a useful blocking agent in this pathway. In this experimental model, sulfasalazine prevented cellular death after UV irradiation. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier Science | - |
dc.relation.isPartOf | JOURNAL OF CATARACT AND REFRACTIVE SURGERY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis* | - |
dc.subject.MESH | Cell Survival | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Electrophoretic Mobility Shift Assay | - |
dc.subject.MESH | Epithelial Cells/metabolism | - |
dc.subject.MESH | Epithelial Cells/pathology | - |
dc.subject.MESH | Epithelial Cells/radiation effects* | - |
dc.subject.MESH | Fluorescent Antibody Technique, Indirect | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Lens, Crystalline/cytology* | - |
dc.subject.MESH | Microscopy, Fluorescence | - |
dc.subject.MESH | NF-kappa B/antagonists & inhibitors | - |
dc.subject.MESH | NF-kappa B/metabolism* | - |
dc.subject.MESH | Sulfasalazine/pharmacology | - |
dc.subject.MESH | Ultraviolet Rays* | - |
dc.title | Cellular death mediated by nuclear factor kappa B (NF-κB) translocation in cultured human lens epithelial cells after ultraviolet-B irradiation | - |
dc.type | Article | - |
dc.publisher.location | United States | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.googleauthor | Do Hyung Lee | - |
dc.contributor.googleauthor | Kyung Sun Cho | - |
dc.contributor.googleauthor | Sang-Gyu Park | - |
dc.contributor.googleauthor | Eung Kwon Kim | - |
dc.contributor.googleauthor | Choun-Ki Joo | - |
dc.identifier.doi | 10.1016/j.jcrs.2004.05.053 | - |
dc.contributor.localId | A00831 | - |
dc.relation.journalcode | J01299 | - |
dc.identifier.eissn | 1873-4502 | - |
dc.identifier.pmid | 15811753 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0886335004006571 | - |
dc.subject.keyword | 15811753 | - |
dc.contributor.alternativeName | Kim, Eung Kweon | - |
dc.citation.volume | 31 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 614 | - |
dc.citation.endPage | 619 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CATARACT AND REFRACTIVE SURGERY, Vol.31(3) : 614-619, 2005 | - |
dc.date.modified | 2017-05-04 | - |
dc.identifier.rimsid | 45213 | - |
dc.type.rims | ART | - |
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