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Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo.

 June Seok Heo  ;  Hyun Ok Kim  ;  Seung Yong Song  ;  Dae Hyun Lew  ;  Youjeong Choi  ;  Sinyoung Kim 
 BIOMED RESEARCH INTERNATIONAL, Vol.2016 : 8196078, 2016 
Journal Title
Issue Date
Adipocytes/cytology ; Cell Culture Techniques* ; Cell Cycle ; Cell Differentiation ; Cell Lineage ; Cell Proliferation ; Cells, Cultured ; Cellular Senescence/drug effects* ; Extracellular Matrix/metabolism ; Flow Cytometry ; Genome, Human ; Humans ; Immunophenotyping ; Mesenchymal Stromal Cells/cytology* ; Oligonucleotide Array Sequence Analysis ; Osteogenesis ; Polylysine/chemistry* ; Regenerative Medicine ; S Phase ; beta-Galactosidase/metabolism
Mesenchymal stem cells (MSCs) possess great therapeutic potential. Efficient in vitro expansion of MSCs is however necessary for their clinical application. The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, and it has been used as a coating substrate for cell culture. In this study, we have aimed to improve the functionality and stemness of MSCs during culture using poly-L-lysine (PLL). Functionality of MSCs was analysed by cell cycle analysis, differentiation assay, β-galactosidase staining, and RT-PCR. Furthermore, we assessed the global gene expression profile of MSCs on uncoated and PLL-coated plates. MSCs on PLL-coated plates exhibited a faster growth rate with increased S-phase and upregulated expression of the stemness markers. In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. We also confirmed that MSCs on uncoated plates expressed higher β-galactosidase than the MSCs on PLL-coated plates. We demonstrate that PLL provides favourable microenvironment for MSC culture by reversing the replicative senescence. This method will significantly contribute to effective preparation of MSCs for cellular therapy.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Song, Seung Yong(송승용) ORCID logo https://orcid.org/0000-0002-3145-7463
Lew, Dae Hyun(유대현)
Choi, You Jeoung(최유정)
Heo, June Seok(허준석)
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