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IgE Reactivity of Recombinant Pac c 3 from the Asian Needle Ant (Pachycondyla chinensis).

 Kyoung Yong Jeong  ;  Myung-hee Yi  ;  Mina Son  ;  Dongpyo Lyu  ;  Jae-Hyun Lee  ;  Tai-Soon Yong  ;  Jung-Won Park 
 International Archives of Allergy and Immunology, Vol.169(2) : 93-100, 2016 
Journal Title
 International Archives of Allergy and Immunology 
Issue Date
Adult ; Allergens/immunology* ; Amino Acid Sequence ; Anaphylaxis/blood ; Anaphylaxis/immunology* ; Animals ; Ants/immunology* ; Case-Control Studies ; Cross Reactions/immunology ; Female ; Humans ; Immunoglobulin E/blood ; Immunoglobulin E/immunology* ; Insect Bites and Stings ; Insect Proteins/chemistry ; Insect Proteins/immunology* ; Male ; Middle Aged ; Molecular Sequence Data ; Recombinant Proteins/chemistry ; Recombinant Proteins/immunology* ; Sequence Alignment ; Young Adult
Ant sting anaphylaxis ; Antigen 5 ; Pac c 3 ; Pachycondyla chinensis
BACKGROUND: Stings from the Asian needle ant are an important cause of anaphylaxis in East Asia. A 23-kDa protein homologous to antigen 5 is the major allergen produced by these ants. In this study, we aimed to produce a recombinant antigen 5 allergen, Pac c 3. METHODS: Recombinant Pac c 3 allergen from the Asian needle ant was expressed in Pichia pastoris and purified by ammonium sulfate precipitation and Ni affinity chromatography. IgE reactivity was demonstrated by ELISA and immunoblotting. RESULTS: The recombinant protein was recognized in 5 of 6 (83.3%) serum samples from patients with demonstrated anaphylaxis to ants. IgE reactivity to an antigen 5 allergen from Asian needle ant venom sac extract was specifically inhibited by the recombinant protein. It was also able to inhibit IgE binding to the vespid allergen Ves v 5 by ImmunoCAP analysis, indicating the presence of cross-reactivity. CONCLUSION: A recombinant Pac c 3, cross-reactive with Ves v 5, from the Asian needle ant was successfully produced in the methylotrophic yeast P. pastoris. This protein could be useful for the development of component-resolved diagnostics.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Yi, Myung Hee(이명희) ORCID logo https://orcid.org/0000-0001-9537-5726
Lee, Jae Hyun(이재현) ORCID logo https://orcid.org/0000-0002-0760-0071
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
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