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IgE Reactivity of Recombinant Pac c 3 from the Asian Needle Ant (Pachycondyla chinensis).

DC Field Value Language
dc.contributor.author박중원-
dc.contributor.author용태순-
dc.contributor.author이명희-
dc.contributor.author이재현-
dc.contributor.author정경용-
dc.date.accessioned2017-02-24T11:31:58Z-
dc.date.available2017-02-24T11:31:58Z-
dc.date.issued2016-
dc.identifier.issn1018-2438-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/146790-
dc.description.abstractBACKGROUND: Stings from the Asian needle ant are an important cause of anaphylaxis in East Asia. A 23-kDa protein homologous to antigen 5 is the major allergen produced by these ants. In this study, we aimed to produce a recombinant antigen 5 allergen, Pac c 3. METHODS: Recombinant Pac c 3 allergen from the Asian needle ant was expressed in Pichia pastoris and purified by ammonium sulfate precipitation and Ni affinity chromatography. IgE reactivity was demonstrated by ELISA and immunoblotting. RESULTS: The recombinant protein was recognized in 5 of 6 (83.3%) serum samples from patients with demonstrated anaphylaxis to ants. IgE reactivity to an antigen 5 allergen from Asian needle ant venom sac extract was specifically inhibited by the recombinant protein. It was also able to inhibit IgE binding to the vespid allergen Ves v 5 by ImmunoCAP analysis, indicating the presence of cross-reactivity. CONCLUSION: A recombinant Pac c 3, cross-reactive with Ves v 5, from the Asian needle ant was successfully produced in the methylotrophic yeast P. pastoris. This protein could be useful for the development of component-resolved diagnostics.-
dc.description.statementOfResponsibilityrestriction-
dc.format.extent93~100-
dc.languageEnglish-
dc.publisherS. Karger-
dc.relation.isPartOfINTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAllergens/immunology*-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnaphylaxis/blood-
dc.subject.MESHAnaphylaxis/immunology*-
dc.subject.MESHAnimals-
dc.subject.MESHAnts/immunology*-
dc.subject.MESHCase-Control Studies-
dc.subject.MESHCross Reactions/immunology-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHImmunoglobulin E/blood-
dc.subject.MESHImmunoglobulin E/immunology*-
dc.subject.MESHInsect Bites and Stings-
dc.subject.MESHInsect Proteins/chemistry-
dc.subject.MESHInsect Proteins/immunology*-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHRecombinant Proteins/chemistry-
dc.subject.MESHRecombinant Proteins/immunology*-
dc.subject.MESHSequence Alignment-
dc.subject.MESHYoung Adult-
dc.titleIgE Reactivity of Recombinant Pac c 3 from the Asian Needle Ant (Pachycondyla chinensis).-
dc.typeArticle-
dc.publisher.locationSwitzerland-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorKyoung Yong Jeong-
dc.contributor.googleauthorMyung-hee Yi-
dc.contributor.googleauthorMina Son-
dc.contributor.googleauthorDongpyo Lyu-
dc.contributor.googleauthorJae-Hyun Lee-
dc.contributor.googleauthorTai-Soon Yong-
dc.contributor.googleauthorJung-Won Park-
dc.identifier.doi10.1159/000444364-
dc.contributor.localIdA01681-
dc.contributor.localIdA02424-
dc.contributor.localIdA02761-
dc.contributor.localIdA03086-
dc.contributor.localIdA03572-
dc.relation.journalcodeJ01074-
dc.identifier.eissn1423-0097-
dc.identifier.pmid27043037-
dc.identifier.urlhttp://www.karger.com/Article/FullText/444364-
dc.subject.keywordAnt sting anaphylaxis-
dc.subject.keywordAntigen 5-
dc.subject.keywordPac c 3-
dc.subject.keywordPachycondyla chinensis-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.alternativeNameYi, Myung Hee-
dc.contributor.alternativeNameLee, Jae Hyun-
dc.contributor.alternativeNameJeong, Kyoung Yong-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.contributor.affiliatedAuthorYi, Myung Hee-
dc.contributor.affiliatedAuthorLee, Jae Hyun-
dc.contributor.affiliatedAuthorJeong, Kyoung Yong-
dc.citation.volume169-
dc.citation.number2-
dc.citation.startPage93-
dc.citation.endPage100-
dc.identifier.bibliographicCitationINTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.169(2) : 93-100, 2016-
dc.date.modified2017-02-24-
dc.identifier.rimsid47532-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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