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Designed nucleases for targeted genome editing

Authors
 Junwon Lee  ;  Jae-Hee Chung  ;  Ho Min Kim  ;  Dong-Wook Kim  ;  Hyongbum Kim 
Citation
 PLANT BIOTECHNOLOGY JOURNAL, Vol.14(2) : 448-462, 2016 
Journal Title
PLANT BIOTECHNOLOGY JOURNAL
ISSN
 1467-7644 
Issue Date
2016
MeSH
Base Sequence ; DNA Breaks, Double-Stranded ; DNA Repair/genetics ; Endonucleases/metabolism* ; Gene Editing* ; Genetic Engineering ; Genome, Plant*
Keywords
CRISPR-Cas9 ; double-strand break ; homology-directed repair ; nonhomologous end-joining ; transcription activator-like effector nuclease ; zinc-finger nuclease
Abstract
Targeted genome-editing technology using designed nucleases has been evolving rapidly, and its applications are widely expanding in research, medicine and biotechnology. Using this genome-modifying technology, researchers can precisely and efficiently insert, remove or change specific sequences in various cultured cells, micro-organisms, animals and plants. This genome editing is based on the generation of double-strand breaks (DSBs), repair of which modifies the genome through nonhomologous end-joining (NHEJ) or homology-directed repair (HDR). In addition, designed nickase-induced generation of single-strand breaks can also lead to precise genome editing through HDR, albeit at relatively lower efficiencies than that induced by nucleases. Three kinds of designed nucleases have been used for targeted DSB formation: zinc-finger nucleases, transcription activator-like effector nucleases, and RNA-guided engineered nucleases derived from the bacterial clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated) system. A growing number of researchers are using genome-editing technologies, which have become more accessible and affordable since the discovery and adaptation of CRISPR-Cas9. Here, the repair mechanism and outcomes of DSBs are reviewed and the three types of designed nucleases are discussed with the hope that such understanding will facilitate applications to genome editing.
Files in This Item:
T201600316.pdf Download
DOI
10.1111/pbi.12465
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Wook(김동욱) ORCID logo https://orcid.org/0000-0002-5025-1532
Kim, Hyongbum(김형범) ORCID logo https://orcid.org/0000-0002-4693-738X
Lee, Jun Won(이준원) ORCID logo https://orcid.org/0000-0003-0543-7132
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146366
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