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성장 인자가 배양된 토끼의 심부 굴곡 건 세포에서 제1형 교원질 합성에 미치는 영향

Other Titles
 Effect of Growth Factors on Type I Collagen Synthesis in Cultured Rabbit's Deep Flexor Tendon Cell 
Authors
 신동은  ;  강호정  ;  김현우  ;  강응식  ;  한수봉  ;  김성재  ;  한창동  ;  이우석 
Citation
 Journal of the Korean Orthopaedic Association (대한정형외과학회지), Vol.37(2) : 288-294, 2002 
Journal Title
 Journal of the Korean Orthopaedic Association (대한정형외과학회지) 
ISSN
 1226-2102 
Issue Date
2002
Keywords
건 세포 배양 ; 성장 인자 ; 제 1형 교원질 ; 토끼 굴곡 건 ; Tendon cell culture ; Growth factor ; Type-I collagen ; Rabbit flexor tendon
Abstract
Purpose: This study focuses on the change of the expression of type-Ⅰcollagen mRNA and extent of collagen synthesis caused by four kinds of growth factors (TGF-β, IGF-Ⅰ, EGF, PDGF) and their combinations to elucidate the effects of growth factors on rabbit's flexor tendon. Materials and Methods: Cells were isolated from rabbit's flexor tendons and cultured with DMEM containing 0.5% fetal bovine serum. Growth factors were then given either independently or in combination. On the 5th, 10th, and 15th day, the cells were collected and the cell proliferation rate was analyzed. The expression of type-Ⅰ collagen mRNA was analyzed by Northern blot. Results: The expression of type-Ⅰ collagen mRNA increased significantly in the IGF, PDGF, PDGF/EGF, and PDGF/IGF groups (p<0.05). Western blot analysis showed that the extent of type-Ⅰ collagen synthesis in culture media was similar to the type-Ⅰ collagen mRNA expression. Conclusion: Based on this study, we conclude that PDGF and IGF individually, and the combinations PDGF/IGF, and PDGF/EGF were most effective at aiding the healing process in rabbit's flexor tendon.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Eung Shick(강응식)
Kang, Ho Jung(강호정) ORCID logo https://orcid.org/0000-0003-0273-1264
Kim, Sung Jae(김성재)
Kim, Hyun Woo(김현우) ORCID logo https://orcid.org/0000-0001-8576-1877
Hahn, Soo Bong(한수봉)
Han, Chang Dong(한창동)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144729
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