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Staurosporine mobilizes Ca2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells

Authors
 Y.J. Kim  ;  J.M. An  ;  D.M. Shin  ;  S.I. Lee  ;  H. Sugiya  ;  J.T. Seo 
Citation
 JOURNAL OF DENTAL RESEARCH, Vol.81(11) : 788-793, 2002 
Journal Title
JOURNAL OF DENTAL RESEARCH
ISSN
 0022-0345 
Issue Date
2002
Keywords
Secretory granules ; staurosporine ; submandibular acinar cells ; calcium
Abstract
Staurosporine was previously shown to mobilize Ca2+ from the thapsigargin-insensitive Ca2+ store in rat submandibular acinar cells. However, the nature of the store is not yet known. Therefore, in the present study, the staurosporine-releasable intracellular Ca2+ store was characterized. Staurosporine increased the cytosolic Ca2+ concentration ([Ca2+]c) after the inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ store was depleted. Ionomycin caused only small increases in [Ca2+]c after the depletion of the IP3-sensitive Ca2+ store, whereas ionomycin+monensin caused large increases. However, ionomycin+monensin did not increase [Ca2+]c when added after [Ca2+]c was increased by staurosporine, indicating that the acidic Ca2+ store was the main source of Ca2+. The acidic Ca2+ store appeared to be associated with secretory granules, since ionomycin+monensin- and staurosporine-induced [Ca2+]c increases were significantly reduced when the acinar cells were degranulated. The effect of staurosporine on [Ca2+]c was mimicked by other protein kinase C inhibitors. Therefore, we conclude that staurosporine mobilizes Ca2+ from secretory granules, probably through the inhibition of protein kinase C in rat submandibular acinar cells.
Full Text
http://jdr.sagepub.com/content/81/11/788
DOI
10.1177/154405910208101113
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Seo, Jeong Taeg(서정택) ORCID logo https://orcid.org/0000-0003-2697-0251
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Lee, Syng Ill(이승일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144650
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