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Staurosporine mobilizes Ca2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells
DC Field | Value | Language |
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dc.contributor.author | 서정택 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 이승일 | - |
dc.date.accessioned | 2016-05-16T11:29:01Z | - |
dc.date.available | 2016-05-16T11:29:01Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0022-0345 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/144650 | - |
dc.description.abstract | Staurosporine was previously shown to mobilize Ca2+ from the thapsigargin-insensitive Ca2+ store in rat submandibular acinar cells. However, the nature of the store is not yet known. Therefore, in the present study, the staurosporine-releasable intracellular Ca2+ store was characterized. Staurosporine increased the cytosolic Ca2+ concentration ([Ca2+]c) after the inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ store was depleted. Ionomycin caused only small increases in [Ca2+]c after the depletion of the IP3-sensitive Ca2+ store, whereas ionomycin+monensin caused large increases. However, ionomycin+monensin did not increase [Ca2+]c when added after [Ca2+]c was increased by staurosporine, indicating that the acidic Ca2+ store was the main source of Ca2+. The acidic Ca2+ store appeared to be associated with secretory granules, since ionomycin+monensin- and staurosporine-induced [Ca2+]c increases were significantly reduced when the acinar cells were degranulated. The effect of staurosporine on [Ca2+]c was mimicked by other protein kinase C inhibitors. Therefore, we conclude that staurosporine mobilizes Ca2+ from secretory granules, probably through the inhibition of protein kinase C in rat submandibular acinar cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 788~793 | - |
dc.relation.isPartOf | JOURNAL OF DENTAL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Staurosporine mobilizes Ca2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Y.J. Kim | - |
dc.contributor.googleauthor | J.M. An | - |
dc.contributor.googleauthor | D.M. Shin | - |
dc.contributor.googleauthor | S.I. Lee | - |
dc.contributor.googleauthor | H. Sugiya | - |
dc.contributor.googleauthor | J.T. Seo | - |
dc.identifier.doi | 10.1177/154405910208101113 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01905 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02924 | - |
dc.relation.journalcode | J01367 | - |
dc.identifier.eissn | 1544-0591 | - |
dc.identifier.url | http://jdr.sagepub.com/content/81/11/788 | - |
dc.subject.keyword | Secretory granules | - |
dc.subject.keyword | staurosporine | - |
dc.subject.keyword | submandibular acinar cells | - |
dc.subject.keyword | calcium | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Lee, Syng Ill | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Lee, Syng Ill | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 81 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 788 | - |
dc.citation.endPage | 793 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DENTAL RESEARCH, Vol.81(11) : 788-793, 2002 | - |
dc.identifier.rimsid | 51335 | - |
dc.type.rims | ART | - |
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