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Staurosporine mobilizes Ca2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells

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dc.contributor.author서정택-
dc.contributor.author신동민-
dc.contributor.author이승일-
dc.date.accessioned2016-05-16T11:29:01Z-
dc.date.available2016-05-16T11:29:01Z-
dc.date.issued2002-
dc.identifier.issn0022-0345-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/144650-
dc.description.abstractStaurosporine was previously shown to mobilize Ca2+ from the thapsigargin-insensitive Ca2+ store in rat submandibular acinar cells. However, the nature of the store is not yet known. Therefore, in the present study, the staurosporine-releasable intracellular Ca2+ store was characterized. Staurosporine increased the cytosolic Ca2+ concentration ([Ca2+]c) after the inositol 1,4,5-trisphosphate (IP3)-sensitive Ca2+ store was depleted. Ionomycin caused only small increases in [Ca2+]c after the depletion of the IP3-sensitive Ca2+ store, whereas ionomycin+monensin caused large increases. However, ionomycin+monensin did not increase [Ca2+]c when added after [Ca2+]c was increased by staurosporine, indicating that the acidic Ca2+ store was the main source of Ca2+. The acidic Ca2+ store appeared to be associated with secretory granules, since ionomycin+monensin- and staurosporine-induced [Ca2+]c increases were significantly reduced when the acinar cells were degranulated. The effect of staurosporine on [Ca2+]c was mimicked by other protein kinase C inhibitors. Therefore, we conclude that staurosporine mobilizes Ca2+ from secretory granules, probably through the inhibition of protein kinase C in rat submandibular acinar cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent788~793-
dc.relation.isPartOfJOURNAL OF DENTAL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleStaurosporine mobilizes Ca2+ from secretory granules by inhibiting protein kinase C in rat submandibular acinar cells-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorY.J. Kim-
dc.contributor.googleauthorJ.M. An-
dc.contributor.googleauthorD.M. Shin-
dc.contributor.googleauthorS.I. Lee-
dc.contributor.googleauthorH. Sugiya-
dc.contributor.googleauthorJ.T. Seo-
dc.identifier.doi10.1177/154405910208101113-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01905-
dc.contributor.localIdA02091-
dc.contributor.localIdA02924-
dc.relation.journalcodeJ01367-
dc.identifier.eissn1544-0591-
dc.identifier.urlhttp://jdr.sagepub.com/content/81/11/788-
dc.subject.keywordSecretory granules-
dc.subject.keywordstaurosporine-
dc.subject.keywordsubmandibular acinar cells-
dc.subject.keywordcalcium-
dc.contributor.alternativeNameSeo, Jeong Taeg-
dc.contributor.alternativeNameShin, Dong Min-
dc.contributor.alternativeNameLee, Syng Ill-
dc.contributor.affiliatedAuthorSeo, Jeong Taeg-
dc.contributor.affiliatedAuthorShin, Dong Min-
dc.contributor.affiliatedAuthorLee, Syng Ill-
dc.rights.accessRightsnot free-
dc.citation.volume81-
dc.citation.number11-
dc.citation.startPage788-
dc.citation.endPage793-
dc.identifier.bibliographicCitationJOURNAL OF DENTAL RESEARCH, Vol.81(11) : 788-793, 2002-
dc.identifier.rimsid51335-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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