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Cloning and characterization of a major surface protein (MspTL) of Treponema lecithinolyticum associated with rapidly progressive periodontitis

Authors
 Kwang Kyun Park  ;  Klaus Heuner  ;  Ulf B. Göbel  ;  Yun Jung Yoo  ;  Chong Kwan Kim  ;  Bong Kyu Choi 
Citation
 FEMS MICROBIOLOGY LETTERS, Vol.207(2) : 185-192, 2002 
Journal Title
FEMS MICROBIOLOGY LETTERS
ISSN
 0378-1097 
Issue Date
2002
MeSH
Amino Acid Sequence ; Bacterial Outer Membrane Proteins/chemistry ; Bacterial Outer Membrane Proteins/genetics* ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics* ; Cloning, Molecular ; Genes, Bacterial* ; Humans ; Membrane Proteins/chemistry ; Membrane Proteins/genetics ; Molecular Sequence Data ; Molecular Weight ; Periodontitis/microbiology* ; Porins/chemistry ; Porins/genetics ; Sequence Alignment ; Treponema/chemistry ; Treponema/genetics*
Keywords
Outer membrane protein ; Oral Treponema ; Periodontitis ; Treponema lecithinolyticum
Abstract
The gene encoding a major surface protein (MspTL) of Treponema lecithinolyticum, a periodontopathogen, was cloned and sequenced. The mspTL gene has a 1770-bp open reading frame (ORF) encoding a protein of 590 amino acids with a predicted molecular mass of 65 kDa which had a typical prokaryotic signal sequence (19 amino acids). MspTL showed a high level of homology with major sheath protein (MspA) of Treponema maltophilum, phylogenetically the closest relative of T. lecithinolyticum. Southern blot analysis indicated that the mspTL gene exists in a single copy and Northern blot analysis showed that the mspTL transcript is monocistronic. Another ORF located downstream of mspTL was in the same orientation and encoded a putative protein, in which the first N-terminal 291 amino acids were identified. The homologous region of this protein is also a part on the T. maltophilum mspA locus.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2002.tb11049.x/abstract
DOI
10.1111/j.1574-6968.2002.tb11049.x
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chong Kwan(김종관)
Park, Kwang Kyun(박광균)
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144596
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