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Viscoelastic protection from endothelial damage by air bubbles

Authors
 Eung Kweon Kim  ;  Stephen M. Cristol  ;  Shin J. Kang  ;  Henry F. Edelhauser  ;  Hyung Lae Kim  ;  Jae Bum Lee 
Citation
 JOURNAL OF CATARACT AND REFRACTIVE SURGERY, Vol.28(6) : 1047-1053, 2002 
Journal Title
 JOURNAL OF CATARACT AND REFRACTIVE SURGERY 
ISSN
 0886-3350 
Issue Date
2002
MeSH
Actins/metabolism ; Adolescent ; Adult ; Air* ; Animals ; Child ; Chondroitin/therapeutic use* ; Chondroitin Sulfates ; Drug Combinations ; Endothelium, Corneal/injuries* ; Endothelium, Corneal/metabolism ; Endothelium, Corneal/ultrastructure ; Eye Injuries/metabolism ; Eye Injuries/pathology ; Eye Injuries/prevention & control* ; Humans ; Hyaluronic Acid/therapeutic use* ; Microscopy, Electron, Scanning ; Middle Aged ; Phacoemulsification/methods ; Rabbits
Abstract
Purpose: To determine whether viscoelastic materials effectively protect the corneal endothelium from air bubbles. Setting: Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Korea. Methods: Human eye-bank and rabbit eyes had a standardized phacoemulsification procedure with or without viscoelastic material (Healon® [sodium hyaluronate 1.0%], Healon GV® [sodium hyaluronate 1.4%], or Viscoat® [chondroitin sulfate 4.0%−sodium hyaluronate 3.0%]). The integrity of the endothelium was examined after the procedure with F-actin staining and scanning electron microscopy. Rabbit eyes with and without viscoelastic material (Healon or Viscoat) had a standardized irrigation/aspiration (I/A) procedure. The mucinous layer of the endothelium was examined after the procedure with transmission electron microscopy. Results: In the phacoemulsification experiment without viscoelastic material, with Healon, and with Healon GV, the endothelium of human and rabbit corneas had many areas of cell loss in a pattern consistent with air-bubble damage. With Viscoat, endothelial cells remained intact. In the I/A experiment, the mucinous layer of Viscoat-exposed rabbit endothelium appeared thinner. In the same experiments without viscoelastic material or with Healon, the mucinous layer of the endothelium appeared normal. Conclusions: Viscoat effectively protected the endothelium from air-bubble damage. Viscoat appears to protect the endothelium by acting as a physical barrier. Its adherence is probably related to the way it interacts with the mucinous layer of the endothelium.
Full Text
http://www.sciencedirect.com/science/article/pii/S0886335001013190
DOI
10.1016/S0886-3350(01)01319-0
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144096
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