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Radiation sensitivity depends on OGG1 activity status in human leukemia cell lines.

Authors
 Jin Won Hyun  ;  Gi Jeong Cheon  ;  Hyun Sook Kim  ;  Yun Sil Lee  ;  Eun Young Choi  ;  Byung Hak Yoon  ;  Jeong Soon Kim  ;  Myung Hee Chung 
Citation
 FREE RADICAL BIOLOGY AND MEDICINE, Vol.32(3) : 212-220, 2002 
Journal Title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN
 0891-5849 
Issue Date
2002
MeSH
Apoptosis/radiation effects ; Blotting, Western ; CDC2 Protein Kinase/metabolism ; Cell Cycle/radiation effects ; Cell Division/radiation effects ; Cell Line ; Cell Survival/radiation effects ; Cyclin B/metabolism ; Cyclin B1 ; DNA Damage/radiation effects ; DNA-Formamidopyrimidine Glycosylase ; Dose-Response Relationship, Radiation ; Flow Cytometry ; Gamma Rays ; Humans ; Jurkat Cells ; Leukemia/enzymology* ; Leukemia/genetics ; Lipid Peroxidation/radiation effects ; N-Glycosyl Hydrolases/metabolism* ; Protein-Serine-Threonine Kinases/metabolism ; Radiation Tolerance* ; Time Factors ; Tumor Cells, Cultured
Keywords
8-Hydroxyguanine ; OGG1 ; ApoptosisCyclin B1 ; Cdc2 ; Radiation ; MPM-2-reactive proteins ; Free radicals
Abstract
To assess the role of 8-oxoguanine glycosylase (OGG1) in the cell defense against radiation injury, the radiation-induced cytotoxicities were compared between the mutant type KG-1 featuring a loss of OGG1 activity due to a homozygous mutation of Arg 229 Gln, and the wild type U937. While the following three obvious toxicities were displayed in KG-1, they were observed only minimally in U937. These were: a dramatic arrest at the G2/M phase indicated by a marked increase in both the number of G2/M cells and the expression of cyclin B1, cdc2, and mitotic phosphoprotein monoclonal-2 (MPM-2)-reactive proteins; a severe apoptosis shown by a marked increase in the number of cells with hypo-diploid DNA and DNA fragmentation; and as a result, a severe inhibition of cell growth and proliferation measured by the MTT test and [3H]-thymidine uptake assay. As expected, KG-1 exhibited a significant increase in the 8-hydroxyguanine level in DNA whereas U937 did not. However, the level of irradiation-induced lipid peroxidation was almost the same in both cell lines. All of these symptoms shown by KG-1 were observed in Molt-4 and CEM-CM3, which were also found to feature low OGG1 activity. These findings suggest that OGG1 plays an important role in cell survival from radiation-induced damage and are also indicative of the capability of 8-hydroxyguanine in DNA to induce cellular toxicities.
Full Text
http://www.sciencedirect.com/science/article/pii/S0891584901007936
DOI
10.1016/S0891-5849(01)00793-6
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yun Sil(이윤실)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/144095
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