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Radiation sensitivity depends on OGG1 activity status in human leukemia cell lines.

DC FieldValueLanguage
dc.contributor.author이윤실-
dc.date.accessioned2016-05-16T11:14:09Z-
dc.date.available2016-05-16T11:14:09Z-
dc.date.issued2002-
dc.identifier.issn0891-5849-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/144095-
dc.description.abstractTo assess the role of 8-oxoguanine glycosylase (OGG1) in the cell defense against radiation injury, the radiation-induced cytotoxicities were compared between the mutant type KG-1 featuring a loss of OGG1 activity due to a homozygous mutation of Arg 229 Gln, and the wild type U937. While the following three obvious toxicities were displayed in KG-1, they were observed only minimally in U937. These were: a dramatic arrest at the G2/M phase indicated by a marked increase in both the number of G2/M cells and the expression of cyclin B1, cdc2, and mitotic phosphoprotein monoclonal-2 (MPM-2)-reactive proteins; a severe apoptosis shown by a marked increase in the number of cells with hypo-diploid DNA and DNA fragmentation; and as a result, a severe inhibition of cell growth and proliferation measured by the MTT test and [3H]-thymidine uptake assay. As expected, KG-1 exhibited a significant increase in the 8-hydroxyguanine level in DNA whereas U937 did not. However, the level of irradiation-induced lipid peroxidation was almost the same in both cell lines. All of these symptoms shown by KG-1 were observed in Molt-4 and CEM-CM3, which were also found to feature low OGG1 activity. These findings suggest that OGG1 plays an important role in cell survival from radiation-induced damage and are also indicative of the capability of 8-hydroxyguanine in DNA to induce cellular toxicities.-
dc.description.statementOfResponsibilityopen-
dc.format.extent212~220-
dc.relation.isPartOfFREE RADICAL BIOLOGY AND MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHApoptosis/radiation effects-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCDC2 Protein Kinase/metabolism-
dc.subject.MESHCell Cycle/radiation effects-
dc.subject.MESHCell Division/radiation effects-
dc.subject.MESHCell Line-
dc.subject.MESHCell Survival/radiation effects-
dc.subject.MESHCyclin B/metabolism-
dc.subject.MESHCyclin B1-
dc.subject.MESHDNA Damage/radiation effects-
dc.subject.MESHDNA-Formamidopyrimidine Glycosylase-
dc.subject.MESHDose-Response Relationship, Radiation-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHGamma Rays-
dc.subject.MESHHumans-
dc.subject.MESHJurkat Cells-
dc.subject.MESHLeukemia/enzymology*-
dc.subject.MESHLeukemia/genetics-
dc.subject.MESHLipid Peroxidation/radiation effects-
dc.subject.MESHN-Glycosyl Hydrolases/metabolism*-
dc.subject.MESHProtein-Serine-Threonine Kinases/metabolism-
dc.subject.MESHRadiation Tolerance*-
dc.subject.MESHTime Factors-
dc.subject.MESHTumor Cells, Cultured-
dc.titleRadiation sensitivity depends on OGG1 activity status in human leukemia cell lines.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiation Oncology (방사선종양학)-
dc.contributor.googleauthorJin Won Hyun-
dc.contributor.googleauthorGi Jeong Cheon-
dc.contributor.googleauthorHyun Sook Kim-
dc.contributor.googleauthorYun Sil Lee-
dc.contributor.googleauthorEun Young Choi-
dc.contributor.googleauthorByung Hak Yoon-
dc.contributor.googleauthorJeong Soon Kim-
dc.contributor.googleauthorMyung Hee Chung-
dc.identifier.doi10.1016/S0891-5849(01)00793-6-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03021-
dc.relation.journalcodeJ00906-
dc.identifier.eissn1873-4596-
dc.identifier.pmid11827746-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0891584901007936-
dc.subject.keyword8-Hydroxyguanine-
dc.subject.keywordOGG1-
dc.subject.keywordApoptosisCyclin B1-
dc.subject.keywordCdc2-
dc.subject.keywordRadiation-
dc.subject.keywordMPM-2-reactive proteins-
dc.subject.keywordFree radicals-
dc.contributor.alternativeNameLee, Yun Sil-
dc.contributor.affiliatedAuthorLee, Yun Sil-
dc.rights.accessRightsnot free-
dc.citation.volume32-
dc.citation.number3-
dc.citation.startPage212-
dc.citation.endPage220-
dc.identifier.bibliographicCitationFREE RADICAL BIOLOGY AND MEDICINE, Vol.32(3) : 212-220, 2002-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers

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