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Daunomycin binding to deoxypolynucleotides with alternating sequences: complete thermodynamic profiles of heterogeneous binding sites.

Authors
 Suh, Dongchul  ;  Oh, Yu-Kyoung  ;  Hur, Man-Wook  ;  Ahn, Byungchan  ;  Chaires, Jonathan B. 
Citation
 NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, Vol.21(10) : 637-649, 2002 
Journal Title
 NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS 
ISSN
 1525-7770 
Issue Date
2002
MeSH
Antibiotics, Antineoplastic/chemistry ; Antibiotics, Antineoplastic/metabolism* ; Base Composition ; Base Sequence ; Binding Sites ; Calorimetry/methods ; DNA/chemistry ; DNA/metabolism* ; Daunorubicin/analogs & derivatives ; Daunorubicin/chemistry ; Daunorubicin/metabolism* ; Polydeoxyribonucleotides/chemistry ; Polydeoxyribonucleotides/metabolism* ; Repetitive Sequences, Nucleic Acid ; Spectrometry, Fluorescence ; Thermodynamics ; Titrimetry/methods
Keywords
Alternating sequences ; Anthracyclines ; Daunomycin ; Thermodynamic profiles
Abstract
Complete thermodynamic binding profiles for the interaction of the anticancer drug, daunomycin with natural DNA and synthetic deoxypolynucleotides were described. Fluorescence titration method was used to estimate the equilibrium binding constants. Binding isotherms were found to be surprisingly complex in some cases, presumably because there were heterogeneous sites even in simple deoxypolynucleotides of repeating sequence. Some polynucleotides consisting of alternating sequence contain at least two different binding sites for daunomycin. The binding affinity of the primary binding sites of alternating and non-alternating sequences was found to differ by two orders of magnitude. An isothermal microtitration calorimeter was used to directly measure the binding enthalpy at 25 degrees C with a high sensitivity. The binding enthalpy of poly[d(A-T)] was found to be -5.5 Kcal/mol, which was much lower than any other polynucleotides, while the binding constant of the high affinity sites, was similar. In this report, the complete thermodynamic profiles of daunomycin binding to deoxypolynucleotides were reliably shown for the first time.
Full Text
http://search.ebscohost.com/login.aspx?direct=true&db=mdc&AN=EPTOC7918910&site=ehost-live
DOI
10.1081/NCN-120015722
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143740
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