Cited 9 times in
Daunomycin binding to deoxypolynucleotides with alternating sequences: complete thermodynamic profiles of heterogeneous binding sites.
DC Field | Value | Language |
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dc.contributor.author | 허만욱 | - |
dc.date.accessioned | 2016-05-16T11:04:46Z | - |
dc.date.available | 2016-05-16T11:04:46Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1525-7770 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143740 | - |
dc.description.abstract | Complete thermodynamic binding profiles for the interaction of the anticancer drug, daunomycin with natural DNA and synthetic deoxypolynucleotides were described. Fluorescence titration method was used to estimate the equilibrium binding constants. Binding isotherms were found to be surprisingly complex in some cases, presumably because there were heterogeneous sites even in simple deoxypolynucleotides of repeating sequence. Some polynucleotides consisting of alternating sequence contain at least two different binding sites for daunomycin. The binding affinity of the primary binding sites of alternating and non-alternating sequences was found to differ by two orders of magnitude. An isothermal microtitration calorimeter was used to directly measure the binding enthalpy at 25 degrees C with a high sensitivity. The binding enthalpy of poly[d(A-T)] was found to be -5.5 Kcal/mol, which was much lower than any other polynucleotides, while the binding constant of the high affinity sites, was similar. In this report, the complete thermodynamic profiles of daunomycin binding to deoxypolynucleotides were reliably shown for the first time. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 637~649 | - |
dc.relation.isPartOf | NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Antibiotics, Antineoplastic/chemistry | - |
dc.subject.MESH | Antibiotics, Antineoplastic/metabolism* | - |
dc.subject.MESH | Base Composition | - |
dc.subject.MESH | BaseSequence | - |
dc.subject.MESH | BindingSites | - |
dc.subject.MESH | Calorimetry/methods | - |
dc.subject.MESH | DNA/chemistry | - |
dc.subject.MESH | DNA/metabolism* | - |
dc.subject.MESH | Daunorubicin/analogs & derivatives | - |
dc.subject.MESH | Daunorubicin/chemistry | - |
dc.subject.MESH | Daunorubicin/metabolism* | - |
dc.subject.MESH | Polydeoxyribonucleotides/chemistry | - |
dc.subject.MESH | Polydeoxyribonucleotides/metabolism* | - |
dc.subject.MESH | RepetitiveSequences, Nucleic Acid | - |
dc.subject.MESH | Spectrometry, Fluorescence | - |
dc.subject.MESH | Thermodynamics | - |
dc.subject.MESH | Titrimetry/methods | - |
dc.title | Daunomycin binding to deoxypolynucleotides with alternating sequences: complete thermodynamic profiles of heterogeneous binding sites. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Suh, Dongchul | - |
dc.contributor.googleauthor | Oh, Yu-Kyoung | - |
dc.contributor.googleauthor | Hur, Man-Wook | - |
dc.contributor.googleauthor | Ahn, Byungchan | - |
dc.contributor.googleauthor | Chaires, Jonathan B. | - |
dc.identifier.doi | 10.1081/NCN-120015722 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04350 | - |
dc.relation.journalcode | J02388 | - |
dc.identifier.eissn | 1532-2335 | - |
dc.identifier.pmid | 12502280 | - |
dc.identifier.url | http://search.ebscohost.com/login.aspx?direct=true&db=mdc&AN=EPTOC7918910&site=ehost-live | - |
dc.subject.keyword | Alternating sequences | - |
dc.subject.keyword | Anthracyclines | - |
dc.subject.keyword | Daunomycin | - |
dc.subject.keyword | Thermodynamic profiles | - |
dc.contributor.alternativeName | Hur, Man Wook | - |
dc.contributor.affiliatedAuthor | Hur, Man Wook | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 21 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 637 | - |
dc.citation.endPage | 649 | - |
dc.identifier.bibliographicCitation | NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, Vol.21(10) : 637-649, 2002 | - |
dc.identifier.rimsid | 38635 | - |
dc.type.rims | ART | - |
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