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Identification of radiation specific response from gene expression profile

Authors
 Woong Yang Park  ;  Chang Il Hwang  ;  Chang Nim Im  ;  Min Ji Kang  ;  Jang Hee Woo  ;  Ju Hoon Kim  ;  Yon Su Kim  ;  Ju Han Kim  ;  Ho Kim  ;  Kyung A Kim  ;  Hyung Jin Yu  ;  Sue Jae Lee  ;  Yun Sil Lee  ;  Jeong Sun Seo 
Citation
 ONCOGENE, Vol.21(55) : 8521-8528, 2002 
Journal Title
 ONCOGENE 
ISSN
 0950-9232 
Issue Date
2002
MeSH
Apoptosis ; CD3 Complex/immunology ; Cell Division ; Concanavalin A/pharmacology ; Gene Expression Profiling* ; Gene Expression Regulation, Neoplastic/radiation effects* ; Humans ; Jurkat Cells ; Oligonucleotide Array Sequence Analysis ; Phytohemagglutinins/pharmacology ; Radiation, Ionizing ; Signal Transduction ; T-Lymphocytes/drug effects ; T-Lymphocytes/immunology ; T-Lymphocytes/radiation effects
Keywords
g-irradiation ; cDNA microarray ; p53 ; cytoplasmic epoxide hydrolase ; phospholipase Cg2
Abstract
The responses to ionizing radiation (IR) in tumors are dependent on cellular context. We investigated radiation-related expression patterns in Jurkat T cells with nonsense mutation in p53 using cDNA microarray. Expression of 2400 genes in bold gamma-irradiated cells was distinct from other stimulations like anti-CD3, phetohemagglutinin (PHA) and concanavalin A (ConA) in unsupervised clustering analysis. Among them, 384 genes were selected for their IR-specific changes to make 'RadChip'. In spite of p53 status, every type of cells showed similar patterns in expression of these genes upon bold gamma-radiation. Moreover, radiation-induced responses were clearly separated from the responses to other genotoxic stress like UV radiation, cisplatin and doxorubicin. We focused on two IR-related genes, phospholipase Cbold gamma2 (PLCG2) and cytosolic epoxide hydrolase (EPHX2), which were increased at 12 h after bold gamma-radiation in RT-PCR. TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-kappaB upon bold gamma-radiation. From these results, we could identify the IR-specific genes from expression profiling, which can be used as radiation biomarkers to screen radiation exposure as well as probing the mechanism of cellular responses to ionizing radiation.
Full Text
http://www.nature.com/onc/journal/v21/n55/full/1205977a.html
DOI
10.1038/sj.onc.1205977
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yun Sil(이윤실)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143734
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