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Identification of radiation specific response from gene expression profile

 Woong Yang Park  ;  Chang Il Hwang  ;  Chang Nim Im  ;  Min Ji Kang  ;  Jang Hee Woo  ;  Ju Hoon Kim  ;  Yon Su Kim  ;  Ju Han Kim  ;  Ho Kim  ;  Kyung A Kim  ;  Hyung Jin Yu  ;  Sue Jae Lee  ;  Yun Sil Lee  ;  Jeong Sun Seo 
 ONCOGENE, Vol.21(55) : 8521-8528, 2002 
Journal Title
Issue Date
Apoptosis ; CD3 Complex/immunology ; Cell Division ; Concanavalin A/pharmacology ; Gene Expression Profiling* ; Gene Expression Regulation, Neoplastic/radiation effects* ; Humans ; Jurkat Cells ; Oligonucleotide Array Sequence Analysis ; Phytohemagglutinins/pharmacology ; Radiation, Ionizing ; Signal Transduction ; T-Lymphocytes/drug effects ; T-Lymphocytes/immunology ; T-Lymphocytes/radiation effects
g-irradiation ; cDNA microarray ; p53 ; cytoplasmic epoxide hydrolase ; phospholipase Cg2
The responses to ionizing radiation (IR) in tumors are dependent on cellular context. We investigated radiation-related expression patterns in Jurkat T cells with nonsense mutation in p53 using cDNA microarray. Expression of 2400 genes in bold gamma-irradiated cells was distinct from other stimulations like anti-CD3, phetohemagglutinin (PHA) and concanavalin A (ConA) in unsupervised clustering analysis. Among them, 384 genes were selected for their IR-specific changes to make 'RadChip'. In spite of p53 status, every type of cells showed similar patterns in expression of these genes upon bold gamma-radiation. Moreover, radiation-induced responses were clearly separated from the responses to other genotoxic stress like UV radiation, cisplatin and doxorubicin. We focused on two IR-related genes, phospholipase Cbold gamma2 (PLCG2) and cytosolic epoxide hydrolase (EPHX2), which were increased at 12 h after bold gamma-radiation in RT-PCR. TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-kappaB upon bold gamma-radiation. From these results, we could identify the IR-specific genes from expression profiling, which can be used as radiation biomarkers to screen radiation exposure as well as probing the mechanism of cellular responses to ionizing radiation.
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1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Lee, Yun Sil(이윤실)
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