Cited 104 times in
Identification of radiation specific response from gene expression profile
DC Field | Value | Language |
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dc.contributor.author | 이윤실 | - |
dc.date.accessioned | 2016-05-16T11:04:37Z | - |
dc.date.available | 2016-05-16T11:04:37Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0950-9232 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/143734 | - |
dc.description.abstract | The responses to ionizing radiation (IR) in tumors are dependent on cellular context. We investigated radiation-related expression patterns in Jurkat T cells with nonsense mutation in p53 using cDNA microarray. Expression of 2400 genes in bold gamma-irradiated cells was distinct from other stimulations like anti-CD3, phetohemagglutinin (PHA) and concanavalin A (ConA) in unsupervised clustering analysis. Among them, 384 genes were selected for their IR-specific changes to make 'RadChip'. In spite of p53 status, every type of cells showed similar patterns in expression of these genes upon bold gamma-radiation. Moreover, radiation-induced responses were clearly separated from the responses to other genotoxic stress like UV radiation, cisplatin and doxorubicin. We focused on two IR-related genes, phospholipase Cbold gamma2 (PLCG2) and cytosolic epoxide hydrolase (EPHX2), which were increased at 12 h after bold gamma-radiation in RT-PCR. TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-kappaB upon bold gamma-radiation. From these results, we could identify the IR-specific genes from expression profiling, which can be used as radiation biomarkers to screen radiation exposure as well as probing the mechanism of cellular responses to ionizing radiation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 8521~8528 | - |
dc.relation.isPartOf | ONCOGENE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | CD3 Complex/immunology | - |
dc.subject.MESH | Cell Division | - |
dc.subject.MESH | Concanavalin A/pharmacology | - |
dc.subject.MESH | GeneExpressionProfiling* | - |
dc.subject.MESH | GeneExpressionRegulation, Neoplastic/radiationeffects* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Jurkat Cells | - |
dc.subject.MESH | Oligonucleotide Array Sequence Analysis | - |
dc.subject.MESH | Phytohemagglutinins/pharmacology | - |
dc.subject.MESH | Radiation, Ionizing | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | T-Lymphocytes/drug effects | - |
dc.subject.MESH | T-Lymphocytes/immunology | - |
dc.subject.MESH | T-Lymphocytes/radiationeffects | - |
dc.title | Identification of radiation specific response from gene expression profile | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Radiation Oncology (방사선종양학) | - |
dc.contributor.googleauthor | Woong Yang Park | - |
dc.contributor.googleauthor | Chang Il Hwang | - |
dc.contributor.googleauthor | Chang Nim Im | - |
dc.contributor.googleauthor | Min Ji Kang | - |
dc.contributor.googleauthor | Jang Hee Woo | - |
dc.contributor.googleauthor | Ju Hoon Kim | - |
dc.contributor.googleauthor | Yon Su Kim | - |
dc.contributor.googleauthor | Ju Han Kim | - |
dc.contributor.googleauthor | Ho Kim | - |
dc.contributor.googleauthor | Kyung A Kim | - |
dc.contributor.googleauthor | Hyung Jin Yu | - |
dc.contributor.googleauthor | Sue Jae Lee | - |
dc.contributor.googleauthor | Yun Sil Lee | - |
dc.contributor.googleauthor | Jeong Sun Seo | - |
dc.identifier.doi | 10.1038/sj.onc.1205977 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03021 | - |
dc.relation.journalcode | J02413 | - |
dc.identifier.eissn | 1476-5594 | - |
dc.identifier.pmid | 12466973 | - |
dc.identifier.url | http://www.nature.com/onc/journal/v21/n55/full/1205977a.html | - |
dc.subject.keyword | g-irradiation | - |
dc.subject.keyword | cDNA microarray | - |
dc.subject.keyword | p53 | - |
dc.subject.keyword | cytoplasmic epoxide hydrolase | - |
dc.subject.keyword | phospholipase Cg2 | - |
dc.contributor.alternativeName | Lee, Yun Sil | - |
dc.contributor.affiliatedAuthor | Lee, Yun Sil | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 21 | - |
dc.citation.number | 55 | - |
dc.citation.startPage | 8521 | - |
dc.citation.endPage | 8528 | - |
dc.identifier.bibliographicCitation | ONCOGENE, Vol.21(55) : 8521-8528, 2002 | - |
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