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Cyclooxygenase-2 expression by transcription factors in Helicobacter pylori-infected gastric epithelial cells: Comparison between HP 99 and NCTC 11637

Authors
 Ji Hye Seo  ;  Hyeyoung Kim  ;  Kyung Hwan Kim 
Citation
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.973 : 477-480, 2002 
Journal Title
 ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 
ISSN
 0077-8923 
Issue Date
2002
MeSH
Adenocarcinoma ; Cyclooxygenase 2 ; Gastric Mucosa/enzymology* ; Gastric Mucosa/microbiology* ; Gene Expression Regulation, Enzymologic/physiology* ; Gene Expression Regulation, Neoplastic ; Genotype ; Helicobacter Infections/enzymology ; Helicobacter Infections/genetics* ; Helicobacter pylori/classification ; Helicobacter pylori/genetics ; Helicobacter pylori/pathogenicity* ; Humans ; Isoenzymes/genetics* ; Korea ; Membrane Proteins ; Prostaglandin-Endoperoxide Synthases/genetics* ; Stomach Neoplasms ; Transcription Factors/metabolism* ; Tumor Cells, Cultured
Abstract
Helicobacter pylori, a gram-negative spiral bacterium, has been associated with chronic gastritis and gastric cancer. HP 99, isolated from the Korean patients, and NCTC 11637, obtained from ATCC, have different genotypes. The present study aims to investigate whether these H. pylori strains show the discrepancy for activating transcription factors (NF-κB, AP-1, C/EBP) and induction of cyclooxygenase-2 (COX-2) gene in gastric epithelial AGS cells. After treatment of H. pylori to AGS cells at the ratio of 300:1, the activation of transcription factors was assessed by electrophoretic mobility shift assay. COX-2 protein was determined by Western blot analysis. The level of 6-keto-prostaglandin F1α (6-keto-PGF1α), a COX-2 product, was measured in the medium by enzyme-linked immunosorbent assay. As a result, both H. pylori strains similarly induced COX-2 expression via activation of NF-κB, not C/EBP, and increased the level of 6-keto-PGF1α. HP 99 showed much higher activation of AP-1 compared to NCTC 11637. NF-κB might play an important role in COX-2 expression in H. pylori-induced gastric inflammation as compared to other transcription factors.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.2002.tb04687.x/abstract
DOI
10.1111/j.1749-6632.2002.tb04687.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143324
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