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Cyclooxygenase-2 expression by transcription factors in Helicobacter pylori-infected gastric epithelial cells: Comparison between HP 99 and NCTC 11637

DC FieldValueLanguage
dc.contributor.author김경환-
dc.date.accessioned2016-05-16T10:52:53Z-
dc.date.available2016-05-16T10:52:53Z-
dc.date.issued2002-
dc.identifier.issn0077-8923-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143324-
dc.description.abstractHelicobacter pylori, a gram-negative spiral bacterium, has been associated with chronic gastritis and gastric cancer. HP 99, isolated from the Korean patients, and NCTC 11637, obtained from ATCC, have different genotypes. The present study aims to investigate whether these H. pylori strains show the discrepancy for activating transcription factors (NF-κB, AP-1, C/EBP) and induction of cyclooxygenase-2 (COX-2) gene in gastric epithelial AGS cells. After treatment of H. pylori to AGS cells at the ratio of 300:1, the activation of transcription factors was assessed by electrophoretic mobility shift assay. COX-2 protein was determined by Western blot analysis. The level of 6-keto-prostaglandin F1α (6-keto-PGF1α), a COX-2 product, was measured in the medium by enzyme-linked immunosorbent assay. As a result, both H. pylori strains similarly induced COX-2 expression via activation of NF-κB, not C/EBP, and increased the level of 6-keto-PGF1α. HP 99 showed much higher activation of AP-1 compared to NCTC 11637. NF-κB might play an important role in COX-2 expression in H. pylori-induced gastric inflammation as compared to other transcription factors.-
dc.description.statementOfResponsibilityopen-
dc.format.extent477~480-
dc.relation.isPartOfANNALS OF THE NEW YORK ACADEMY OF SCIENCES-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenocarcinoma-
dc.subject.MESHCyclooxygenase 2-
dc.subject.MESHGastric Mucosa/enzymology*-
dc.subject.MESHGastric Mucosa/microbiology*-
dc.subject.MESHGene Expression Regulation, Enzymologic/physiology*-
dc.subject.MESHGene Expression Regulation, Neoplastic-
dc.subject.MESHGenotype-
dc.subject.MESHHelicobacter Infections/enzymology-
dc.subject.MESHHelicobacter Infections/genetics*-
dc.subject.MESHHelicobacter pylori/classification-
dc.subject.MESHHelicobacter pylori/genetics-
dc.subject.MESHHelicobacter pylori/pathogenicity*-
dc.subject.MESHHumans-
dc.subject.MESHIsoenzymes/genetics*-
dc.subject.MESHKorea-
dc.subject.MESHMembrane Proteins-
dc.subject.MESHProstaglandin-Endoperoxide Synthases/genetics*-
dc.subject.MESHStomach Neoplasms-
dc.subject.MESHTranscription Factors/metabolism*-
dc.subject.MESHTumor Cells, Cultured-
dc.titleCyclooxygenase-2 expression by transcription factors in Helicobacter pylori-infected gastric epithelial cells: Comparison between HP 99 and NCTC 11637-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorJi Hye Seo-
dc.contributor.googleauthorHyeyoung Kim-
dc.contributor.googleauthorKyung Hwan Kim-
dc.identifier.doi10.1111/j.1749-6632.2002.tb04687.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00311-
dc.relation.journalcodeJ00181-
dc.identifier.eissn1749-6632-
dc.identifier.pmid12485915-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.2002.tb04687.x/abstract-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.affiliatedAuthorKim, Kyung Hwan-
dc.rights.accessRightsnot free-
dc.citation.volume973-
dc.citation.startPage477-
dc.citation.endPage480-
dc.identifier.bibliographicCitationANNALS OF THE NEW YORK ACADEMY OF SCIENCES, Vol.973 : 477-480, 2002-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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