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Iron Chlorin e6 Scavenges Hydroxyl Radical and Protects Human Endothelial Cells against Hydrogen Peroxide Toxicity

Authors
 Je-Wook YU  ;  Sung-Sik YOON  ;  Ryung YANG 
Citation
 BIOLOGICAL & PHARMACEUTICAL BULLETIN, Vol.24(9) : 1053-1059, 2001 
Journal Title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN
 0918-6158 
Issue Date
2001
MeSH
Antioxidants/pharmacology* ; Cell Death/drug effects ; Cell Survival/drug effects ; Cells, Cultured ; DNA Adducts/drug effects ; DNA Damage/drug effects ; Electron Spin Resonance Spectroscopy ; Endothelium, Vascular/cytology ; Endothelium, Vascular/drug effects* ; Free Radical Scavengers/pharmacology* ; Humans ; Hydrogen Peroxide/antagonists & inhibitors* ; Hydrogen Peroxide/toxicity ; Hydroxyl Radical/metabolism* ; Metalloporphyrins/pharmacology* ; Superoxides/metabolism
Keywords
iron chlorin e6 (FeCe6) ; antioxidant ; hydroxyl radical scavenger ; hydrogen peroxide toxicity ; oxidative stress
Abstract
Iron chlorin e6 (FeCe6) has recently been proposed to be potentially antimutagenic and antioxidative. However, the antioxidant property of FeCe6 has not been elucidated in detail. In this study, we investigated the ability of FeCe6 to scavenge hydroxyl radical and to protect biomolecules and mammalian cells from oxidative stress-mediated damage. In electron spin resonance (ESR) experiments, FeCe6 showed excellent hydroxyl radical scavenging activity, whereas its iron-deficient molecule, chlorin e6 (Ce6) showed little effect. FeCe6 also significantly reduced hydroxyl radical-induced thiobarbituric acid reactive substance (TBARS) formation and benzoate hydroxylation in a dose-dependent manner. The rate constant for reaction between FeCe6 and hydroxyl radical was measured as 8.5 x 10(10) M(-1) s(-1) by deoxyribose degradation method, and this value was much higher than that of most hydroxyl radical scavengers. Superoxide dismutase (SOD) activity of FeCe6 was also confirmed by ESR study and cytochrome c reduction assay, but its in vitro activity appeared to be less efficient in comparison with other well-known SOD mimics. In addition, FeCe6 appreciably diminished hydroxyl radical-induced DNA single-strand breakage and protein degradation in Fe-catalyzed and Cu-catalyzed Fenton systems, and it significantly protected human endothelial cells against hydrogen peroxide (H2O2) toxicity. These results suggest that FeCe6 is a novel hydroxyl radical scavenger and may be useful for preventing oxidative injury in biological systems.
Files in This Item:
T200103887.pdf Download
DOI
10.1248/bpb.24.1053
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yu, Je Wook(유제욱) ORCID logo https://orcid.org/0000-0001-5943-4071
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143208
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