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Warm Ischemic Time에 따른 냉동보존돼지판막의 세포생존율

Other Titles
 Cellular Viability of Cryopreserved Porcine Valve According to Warm Ischemic Time 
 박영환  ;  윤치순  ;  이종은  ;  장병철  ;  박종철  ;  서활  ;  조범구 
 Korean Journal of Thoracic and Cardiovascular Surgery (대한흉부외과학회지), Vol.34(4) : 305-310, 2001 
Journal Title
Korean Journal of Thoracic and Cardiovascular Surgery(대한흉부외과학회지)
Issue Date
Transplantation ; homolegous ; Viability ; Cryopreservation
Background: Valve replacement using cryopreserved valved homograft is increasing because of resistance of infection and excellent hemodynamics. The viability of fibroblast which is related with warm ischemic time affects the durability of implanted cryopreserved valved homograft. We evaluated how long the warm schemic time is acceptable by examining the viability of cells depending upon warm ischemic time.
Material and Method: 1. Retrieval of tissues; Thirty-two slaughted porcine heart and lung enblocs were stored at refrigerator(4 ∼8 ℃) for various time period(Warm Ischemic Time), and the heart was dissected and stored in Hartman solution at 4 ℃for 24 hours(Cold Ischemic Time) as the simulation of retrieval and dissection of human heart. The hearts were assigned to groups A(2 hours), B(12 hours), C(24 hours), D(36 hours) depending on warm ischemic time. 2. Sterilization; The valved homografts were sterilized in the RPMI 1640 solution with antibiotics. 3. Freezing and Storage; The homografts were freezed by computerized freezer , stored 7 days at liquid nitrogen tank, and thawed. 4. Evaluation of the viability; The viability was evaluated by Triphan blue test after warm ischemic time, after cold ischemic time and after thawing. 5. Analysis; The viability of fibroblast was analysed by pearson correlation test of SAS program.
Result: 1. The viability between after cold ischemic time and after thawing was not different(p=0.619) for the adequacy of sterilization, freezing and thawing. 2. The viability which was evaluated after warm ischemic time, cold ischemic time and thawing, and the various warm ischemic times are strongly correlated as R is -0.857, -0.673 and -0.549 respectively. The viability of tricuspid valve is well related with the viability of aortic valve.
Conclusion: 1. The longer the warm ischemic time, the lesser the viability of fibroblast. The viability of fibroblast after cryopreservation was decreased less 60% if the warm ischemic time was over 12 hours. 2. The method of cryopreservation is acceptable for maintaining the viability of fibroblast, and the viability of tricuspid valve may be the indicator of the viability of aortic valve. 3. However, the study for the optimal viability which is necessary to the durabiltiy of implanted valved homograft is needed.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Hwan(박영환) ORCID logo https://orcid.org/0000-0001-9802-8017
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
Lee, Jong Eun(이종은)
Chang, Byung Chul(장병철)
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