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사염화탄소에 의해 활성화된 간성상세포에서 G1 cyclin 및 Rb-E2F의 발현과 비타민 E의 효과

Other Titles
 The expression of G1 cyclins and Rb-E2F, and the effect of vitamin E on the hepatic stellate cells activated by CCl4 
Authors
 이광재  ;  이관식  ;  한광협  ;  전재윤  ;  문영명  ;  오상환  ;  김호근 
Citation
 Korean Journal of Gastroenterology, Vol.38(4) : 262-269, 2001 
Journal Title
Korean Journal of Gastroenterology(대한소화기학회지)
ISSN
 1598-9992 
Issue Date
2001
Keywords
hepatic stellate cell ; hepatic fibrosis ; CCl4 ; vitamin E
Abstract
Background/Aims: It is obscure when the activation of hepatic stellate cells and the expression of its related factors occur in acute liver injury. Vitamin E is expected to prevent hepatic fibrosis. The aims of this study were to establish the model of hepatic stellate cell activation in acute liver injury and to confirm the effect of vitamin E for preventing hepatic fibrosis. Methods: Male Sprague-Dawley rats were classified into two groups. The one group received a single injection of CCl4 and the other group received injection of vitamin E daily and a single injection of CCl4. The serial changes of serum ALT, and [3H]thymidine uptake, α-SMA, cyclin D1, CDK4, cyclin E, CDK2, Rb, E2F-1 and NF-κB of stellate cells were measured. Results: The serial changes of serum ALT levels, [3H]thymidine uptake, and α-SMA positive cells showed maximum increase at 32 hours after CCl4 injection. However, they were significantly decreased with injection of vitamin E. CDK4, cyclin E and CDK2 showed definite band at 16, 32, 48 hours after CCl4 injection, which diminished or disappeared with injection of vitamin E. Cyclin D1, Rb, E2F-1 and NF-κB showed definite band at 32 hours after CCl4 injection, which also diminished or disappeared with injection of vitamin E. Conclusions: We established an in vivo model of hepatic stellate cell activation in acute liver injury and confirmed the effect of vitamin E in preventing hepatic fibrosis.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hogeun(김호근)
Lee, Kwan Sik(이관식) ORCID logo https://orcid.org/0000-0002-3672-1198
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142999
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