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Oxidative stress effect on the activation of hepatic stellate cells

Authors
 Kwan Sik Lee  ;  Se Joon Lee  ;  Hyo Jin Park  ;  Jun Pyo Chung  ;  Kwang Hyub Han  ;  Chae Yoon Chon  ;  Sang In Lee  ;  Young Myoung Moon 
Citation
 YONSEI MEDICAL JOURNAL, Vol.42(1) : 1-8, 2001 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
2001
MeSH
Actins/metabolism ; Animals ; Cells, Cultured ; Extracellular Matrix Proteins/metabolism ; Liver/cytology* ; Liver Cirrhosis/etiology* ; Liver Cirrhosis/prevention & control ; Male ; NF-kappa B/metabolism* ; Oxidative Stress* ; Rats ; Rats, Sprague-Dawley ; Vitamin E/pharmacology
Keywords
hepatic stellate cell ; oxidative stress ; NFkB
Abstract
Collagen is the most excessive extracellular matrix protein in hepatic fibrosis. Activated, but not quiescent, hepatic stellate cells (HSCs) have a high level of collagen and a smooth muscle actin (α SMA) expression. HSCs play a key role in the pathogenesis of hepatic fibrosis. We analyzed a mechanism leading to HSC activation by evaluating the role of oxidative stress and the expression of NFkB. In vitro study HSCs were proliferated (PCNA:2% vs 68%) and activated (α SMA: 5% vs 78%) by ascorbate/FeSO4, and HSCs activated by type I collagen were blocked (PCNA: 97% vs 4%, a SMA: 86% vs 9%) by a-tocopherol. In vivo study means of a SMA positive cells in liver at 400 × HPF were 48.3±5.2 and 15.2±1.8 and [3H]thymidine uptake of HSC was 529.2±284.8 cpm and 223.0±86.3 cpm in control and a-tocopherol treated group respectively at 32 hours after CCl4 injection. Nuclear extracts from activated, but not from quiescent, HSCs formed a complex with the NFkB cognate oligonucleotidesand α-tocopherol inhibited this bindings. This study indicates that oxidative stress plays an essential role through the induction of NFkB on HSC activation.
Files in This Item:
T200103425.pdf Download
DOI
10.3349/ymj.2001.42.1.1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Hyo Jin(박효진) ORCID logo https://orcid.org/0000-0003-4814-8330
Lee, Kwan Sik(이관식) ORCID logo https://orcid.org/0000-0002-3672-1198
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/142954
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