Cited 78 times in
Oxidative stress effect on the activation of hepatic stellate cells
DC Field | Value | Language |
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dc.contributor.author | 박효진 | - |
dc.contributor.author | 이관식 | - |
dc.date.accessioned | 2016-02-19T11:21:08Z | - |
dc.date.available | 2016-02-19T11:21:08Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/142954 | - |
dc.description.abstract | Collagen is the most excessive extracellular matrix protein in hepatic fibrosis. Activated, but not quiescent, hepatic stellate cells (HSCs) have a high level of collagen and a smooth muscle actin (α SMA) expression. HSCs play a key role in the pathogenesis of hepatic fibrosis. We analyzed a mechanism leading to HSC activation by evaluating the role of oxidative stress and the expression of NFkB. In vitro study HSCs were proliferated (PCNA:2% vs 68%) and activated (α SMA: 5% vs 78%) by ascorbate/FeSO4, and HSCs activated by type I collagen were blocked (PCNA: 97% vs 4%, a SMA: 86% vs 9%) by a-tocopherol. In vivo study means of a SMA positive cells in liver at 400 × HPF were 48.3±5.2 and 15.2±1.8 and [3H]thymidine uptake of HSC was 529.2±284.8 cpm and 223.0±86.3 cpm in control and a-tocopherol treated group respectively at 32 hours after CCl4 injection. Nuclear extracts from activated, but not from quiescent, HSCs formed a complex with the NFkB cognate oligonucleotidesand α-tocopherol inhibited this bindings. This study indicates that oxidative stress plays an essential role through the induction of NFkB on HSC activation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1~8 | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Actins/metabolism | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Extracellular Matrix Proteins/metabolism | - |
dc.subject.MESH | Liver/cytology* | - |
dc.subject.MESH | Liver Cirrhosis/etiology* | - |
dc.subject.MESH | Liver Cirrhosis/prevention & control | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | NF-kappa B/metabolism* | - |
dc.subject.MESH | Oxidative Stress* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Vitamin E/pharmacology | - |
dc.title | Oxidative stress effect on the activation of hepatic stellate cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Kwan Sik Lee | - |
dc.contributor.googleauthor | Se Joon Lee | - |
dc.contributor.googleauthor | Hyo Jin Park | - |
dc.contributor.googleauthor | Jun Pyo Chung | - |
dc.contributor.googleauthor | Kwang Hyub Han | - |
dc.contributor.googleauthor | Chae Yoon Chon | - |
dc.contributor.googleauthor | Sang In Lee | - |
dc.contributor.googleauthor | Young Myoung Moon | - |
dc.identifier.doi | 10.3349/ymj.2001.42.1.1 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01774 | - |
dc.contributor.localId | A02666 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 11293487 | - |
dc.subject.keyword | hepatic stellate cell | - |
dc.subject.keyword | oxidative stress | - |
dc.subject.keyword | NFkB | - |
dc.contributor.alternativeName | Park, Hyo Jin | - |
dc.contributor.alternativeName | Lee, Kwan Sik | - |
dc.contributor.affiliatedAuthor | Park, Hyo Jin | - |
dc.contributor.affiliatedAuthor | Lee, Kwan Sik | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 42 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 8 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.42(1) : 1-8, 2001 | - |
dc.identifier.rimsid | 38655 | - |
dc.type.rims | ART | - |
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