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형질전환 생쥐에서 인간 혹스 조절인자에 의해 유도된 리포터 transgene의 역동적 발현패턴

Other Titles
 Reporter transgene based on s human HOX regulatory element is expressed dynamically during mouse development 
 신재승  ;  박성도  ;  권윤정  ;  이철상  ;  허만욱  ;  박형우  ;  김명희 
 GENES & GENOMICS, Vol.23(4) : 385-392, 2001 
Journal Title
Issue Date
cis - acting regulatory element ; Hox gene ; spatio - temporal regulation ; transgenic mice
In the previous experiment, we have transiently analyzed a 307 bp cis-acting regulatory element of HOXA-7 in transgenic mice (TG). Since it was sufficient to direct a reporter gene to be expressed at the specific region of day 12.5 post coitum (p.c.) embryo with a sharp anterior boundary, we generated transgenic mice line using the NM307 (307 bp/tk promoter/LacZ/SV40 poly A) construct in order to see the temporal expression pattern following embryonic development. Through polymerase chain reaction (PCR) with LacZ specific primers and genomic DNAs isolated from ear tissue, 4 transgenic mice (founder mice, F0) were obtained and 3 exhibited characteristic expression pattern. The expression detected as early as day 8.5 p.c. in the neuroectoderm and epiblast at the posterior region; from the closed neural tube around the region S10-12 to the adjacent posterior neural fold, neural groove and to the posterior epiblast along the anteroposterior axis. At day 7.5 p.c., however, LacZ expression was not detected. As embryo develops, the expression was restricted in the neural tube from S10 area to the end of tail of day 9.5 p.c. embryo, among which rather low level of expression was detected particularly in the region of S10-13, and the overall expression pattern continued through the following day. From day 11.5 to 12.5 p.c. weak spreading of the expression was detected to the anterior of S10 and a repression at the tail region. These results altogether indicate that the 307 bp is a temporal as well as a spatial regulatory element of HOXA-7 initiating the function at around day 7.5.~8.5 p.c. and continuing through mid gestation.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Yunjeong(권윤정)
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Park, Hyoung Woo(박형우) ORCID logo https://orcid.org/0000-0002-5341-9249
Shin, Jaeseung(신재승) ORCID logo https://orcid.org/0000-0002-6755-4732
Hur, Man Wook(허만욱) ORCID logo https://orcid.org/0000-0002-3416-1334
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