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Hsp70 Knockdown by siRNA Decreased Collagen Production in Keloid Fibroblasts

 Jung U Shin  ;  Won Jai Lee  ;  Thanh-Nga Tran  ;  Inhee Jung  ;  Ju Hee Lee 
 YONSEI MEDICAL JOURNAL, Vol.56(6) : 1619-1626, 2015 
Journal Title
Issue Date
Adolescent ; Adult ; Blotting, Western ; Collagen/drug effects* ; Collagen/metabolism ; Female ; Fibroblasts/metabolism ; Gene Expression Regulation ; HSP70 Heat-Shock Proteins/genetics ; HSP70 Heat-Shock Proteins/metabolism ; HSP70 Heat-Shock Proteins/pharmacology* ; Humans ; Keloid/drug therapy* ; Keloid/genetics ; Keloid/metabolism ; Male ; RNA, Messenger/genetics* ; RNA, Small Interfering/genetics* ; Real-Time Polymerase Chain Reaction ; Transfection ; Up-Regulation
Heat shock proteins ; Hsp70 ; keloids
PURPOSE: There are currently no consistently effective treatments for the excessive collagen produced by keloid fibroblasts. Previously, we reported that heat shock protein 70 (Hsp70) is up-regulated in keloid fibroblasts and keloid tissue. We, therefore, investigated whether Hsp70 is related to excessive collagen production in keloid fibroblasts. MATERIALS AND METHODS: We inhibited Hsp70 in keloid fibroblasts by RNA interference and examined the resulting collagen expression. Thus, we selected small interfering RNAs (siRNAs) specific for human Hsp70, transfected them into keloid fibroblasts, and evaluated the resulting phenotypes and protein production using real-time polymerase chain reaction (PCR), Western blot, and a collagen assay. RESULTS: The siRNAs dramatically suppressed Hsp70 mRNA expression, resulting in a decrease in collagen production in the keloid fibroblasts compared with controls. The siRNAs did not influence the viability of the keloid fibroblasts. CONCLUSION: Hsp70 overexpression likely plays an important role in the excessive collagen production by keloid fibroblasts. RNA interference has therapeutic potential for the treatment of keloids.
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1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Shin, Jung U(신정우) ORCID logo https://orcid.org/0000-0001-5259-6879
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
Jung, Inhee(정인희)
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