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Coculture of Primary Motor Neurons and Schwann Cells as a Model for In Vitro Myelination

Authors
 Sujin Hyung  ;  Bo Yoon Lee  ;  Jong-Chul Park  ;  Jinseok Kim  ;  Eun-Mi Hur  ;  Jun-Kyo Francis Suh 
Citation
 Scientific Reports, Vol.5 : 15122-15122, 2015 
Journal Title
 Scientific Reports 
ISSN
 2045-2322 
Issue Date
2015
Abstract
A culture system that can recapitulate myelination in vitro will not only help us better understand the mechanism of myelination and demyelination, but also find out possible therapeutic interventions for treating demyelinating diseases. Here, we introduce a simple and reproducible myelination culture system using mouse motor neurons (MNs) and Schwann cells (SCs). Dissociated motor neurons are plated on a feeder layer of SCs, which interact with and wrap around the axons of MNs as they differentiate in culture. In our MN-SC coculture system, MNs survived over 3 weeks and extended long axons. Both viability and axon growth of MNs in the coculture were markedly enhanced as compared to those of MN monoculture. Co-labeling of myelin basic proteins (MBPs) and neuronal microtubules revealed that SC formed myelin sheaths by wrapping around the axons of MNs. Furthermore, using the coculture system we found that treatment of an antioxidant substance coenzyme Q10 (Co-Q10) markedly facilitated myelination.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141493
DOI
10.1038/srep15122
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실)
Yonsei Authors
박종철(Park, Jong Chul)
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