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Therapeutic Effect of Resveratrol on Oxidative Stress in Graves' Orbitopathy Orbital Fibroblasts

Authors
 Chang Yeom Kim  ;  Hyun Jung Lee  ;  Min Kyung Chae  ;  Jung Woo Byun  ;  Eun Jig Lee  ;  Jin Sook Yoon 
Citation
 INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.56(11) : 6352-6361, 2015 
Journal Title
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
ISSN
 0146-0404 
Issue Date
2015
MeSH
Adipocytes/metabolism ; Adipocytes/pathology* ; Adipogenesis/drug effects* ; Angiogenesis Inhibitors ; Antioxidants/therapeutic use ; Cell Proliferation ; Cells, Cultured ; Fibroblasts/metabolism ; Fibroblasts/pathology ; Graves Ophthalmopathy/drug therapy* ; Graves Ophthalmopathy/metabolism ; Graves Ophthalmopathy/pathology ; Humans ; Oxidative Stress/drug effects* ; Reactive Oxygen Species/metabolism ; Stilbenes/therapeutic use*
Keywords
adipogenesis ; Graves’ orbitopathy ; orbital fibroblast ; oxidative stress ; reactive oxygen species ; resveratrol
Abstract
PURPOSE: Multiple causative factors complicate the pathogenesis in Graves' orbitopathy (GO). It has been suggested that oxidative stress contributes to the development and progression of GO. Therefore, we investigated the therapeutic effect of resveratrol, a potent antioxidant, upon oxidative stress levels in GO orbital fibroblasts in vitro.

METHODS: Orbital fibroblasts were cultured from orbital connective tissues obtained from GO patients. Intracellular reactive oxygen species (ROS) levels and the expression of heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, and thioredoxin (Trx), were measured after resveratrol treatment. Adipogenesis was induced, and ROS levels were examined during adipogenic differentiation. Western blot assay was performed to evaluate the effects of resveratrol on the expression of antioxidants levels and transcriptional regulators.

RESULTS: Treatment with 30 or 50 μM resveratrol reduced ROS production and HO-1 level induced by oxidative stress. Levels of Cu/Zn-SOD, catalase, and Trx were also reduced, while Mn-SOD increased with 50 μM resveratrol treatment. Resveratrol suppressed adipogenesis, reducing the number of adipocytes and suppressing the accumulation of lipid droplets. Treatment with 50 μM resveratrol also decreased ROS levels during adipogenesis. Expression of the transcriptional regulators phosphor-extracellular signal-regulated kinase and phospho-c-Jun NH(2)-terminal kinase significantly increased after treatment with 50 μM resveratrol, and decreased in response to inhibitors of each protein. Phosphonuclear factor kappa-light-chain-enhancer of activated B cells p65 levels also increased after treatment with 50 μM resveratrol.

CONCLUSIONS: Resveratrol reduced ROS levels and inhibited adipogenesis in GO orbital fibroblasts in vitro. This study supports the potential use of resveratrol in GO treatment.
Full Text
http://iovs.arvojournals.org/article.aspx?articleid=2454873
DOI
10.1167/iovs.15-16870
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Chang Yeom(김창염)
Byun, Jung Woo(변정우)
Yoon, Jin Sook(윤진숙) ORCID logo https://orcid.org/0000-0002-8751-9467
Lee, Eun Jig(이은직) ORCID logo https://orcid.org/0000-0002-9876-8370
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141410
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