0 126

Cited 1 times in

Performance of PCR-reverse blot hybridization assay for detection of rifampicin-resistant Mycobacterium leprae

Authors
 Hyeyoung Wang  ;  Hyunjung Kim  ;  Yeun Kim  ;  Hyeeun Bang  ;  Jong Pill Kim  ;  Joo Hwan Hwang  ;  Sang Nae Cho  ;  Tae Ue Kim  ;  Hyeyoung Lee 
Citation
 Journal of Microbiology, Vol.53(10) : 686-693, 2015 
Journal Title
 Journal of Microbiology 
ISSN
 1225-8873 
Issue Date
2015
MeSH
Amino Acid Sequence ; Antibiotics, Antitubercular/pharmacology* ; Base Sequence ; DNA-Directed RNA Polymerases/genetics ; Drug Resistance, Bacterial ; Humans ; Molecular Sequence Data ; Mutation ; Mycobacterium leprae/drug effects* ; Mycobacterium leprae/genetics* ; Mycobacterium leprae/isolation & purification ; Nucleic Acid Hybridization/methods* ; Polymerase Chain Reaction/methods* ; Rifampin/pharmacology* ; Sensitivity and Specificity
Keywords
Mycobacterium leprae ; PCR-REBA (Reverse blot hybridization assay) ; molecular diagnosis ; rifampicin
Abstract
Drug resistance in Mycobacterium leprae is a significant problem in countries where leprosy is endemic. A sensitive, specific, and high-throughput reverse blot hybridization assay (REBA) for the detection of genotypic resistance to rifampicin (RIF) was designed and evaluated. It has been shown that resistance to RIF in M. leprae involves mutations in the rpoB gene encoding the -subunit of the RNA polymerase. The PCR-REBA simultaneously detects both 6 wild-type regions and 5 different mutations (507AGC, 513GTG, 516TAT, 531ATG, and 531TTC) including the most prevalent mutations at positions 507 and 531. Thirty-one clinical isolates provided by Korea Institute of Hansen-s Disease were analyzed by PCR-REBA with RIF resistance of rpoB gene. As a result, missense mutations at codons 507 AGC and 531ATG with 2-nucleotide substitutions were found in one sample, and a missense mutation at codon 516 TAT and ΔWT6 (deletion of 530-534) was found in another sample. These cases were confirmed by DNA sequence analysis. This rapid, simple, and highly sensitive assay provides a practical alternative to sequencing for genotypic evaluation of RIF resistance in M. leprae
Full Text
http://link.springer.com/article/10.1007/s12275-015-5057-9
DOI
10.1007/s12275-015-5057-9
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sang Nae(조상래)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141397
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse