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Performance of PCR-reverse blot hybridization assay for detection of rifampicin-resistant Mycobacterium leprae

Authors
 Hyeyoung Wang  ;  Hyunjung Kim  ;  Yeun Kim  ;  Hyeeun Bang  ;  Jong Pill Kim  ;  Joo Hwan Hwang  ;  Sang Nae Cho  ;  Tae Ue Kim  ;  Hyeyoung Lee 
Citation
 Journal of Microbiology, Vol.53(10) : 686-693, 2015 
Journal Title
 Journal of Microbiology 
ISSN
 1225-8873 
Issue Date
2015
Abstract
Drug resistance in Mycobacterium leprae is a significant problem in countries where leprosy is endemic. A sensitive, specific, and high-throughput reverse blot hybridization assay (REBA) for the detection of genotypic resistance to rifampicin (RIF) was designed and evaluated. It has been shown that resistance to RIF in M. leprae involves mutations in the rpoB gene encoding the -subunit of the RNA polymerase. The PCR-REBA simultaneously detects both 6 wild-type regions and 5 different mutations (507AGC, 513GTG, 516TAT, 531ATG, and 531TTC) including the most prevalent mutations at positions 507 and 531. Thirty-one clinical isolates provided by Korea Institute of Hansen-s Disease were analyzed by PCR-REBA with RIF resistance of rpoB gene. As a result, missense mutations at codons 507 AGC and 531ATG with 2-nucleotide substitutions were found in one sample, and a missense mutation at codon 516 TAT and ΔWT6 (deletion of 530-534) was found in another sample. These cases were confirmed by DNA sequence analysis. This rapid, simple, and highly sensitive assay provides a practical alternative to sequencing for genotypic evaluation of RIF resistance in M. leprae
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141397
DOI
10.1007/s12275-015-5057-9
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실)
Yonsei Authors
조상래(Cho, Sang Nae)
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Full Text
http://link.springer.com/article/10.1007/s12275-015-5057-9
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