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Performance of PCR-reverse blot hybridization assay for detection of rifampicin-resistant Mycobacterium leprae

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dc.contributor.author조상래-
dc.date.accessioned2016-02-04T11:52:35Z-
dc.date.available2016-02-04T11:52:35Z-
dc.date.issued2015-
dc.identifier.issn1225-8873-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/141397-
dc.description.abstractDrug resistance in Mycobacterium leprae is a significant problem in countries where leprosy is endemic. A sensitive, specific, and high-throughput reverse blot hybridization assay (REBA) for the detection of genotypic resistance to rifampicin (RIF) was designed and evaluated. It has been shown that resistance to RIF in M. leprae involves mutations in the rpoB gene encoding the -subunit of the RNA polymerase. The PCR-REBA simultaneously detects both 6 wild-type regions and 5 different mutations (507AGC, 513GTG, 516TAT, 531ATG, and 531TTC) including the most prevalent mutations at positions 507 and 531. Thirty-one clinical isolates provided by Korea Institute of Hansen-s Disease were analyzed by PCR-REBA with RIF resistance of rpoB gene. As a result, missense mutations at codons 507 AGC and 531ATG with 2-nucleotide substitutions were found in one sample, and a missense mutation at codon 516 TAT and ΔWT6 (deletion of 530-534) was found in another sample. These cases were confirmed by DNA sequence analysis. This rapid, simple, and highly sensitive assay provides a practical alternative to sequencing for genotypic evaluation of RIF resistance in M. leprae-
dc.description.statementOfResponsibilityopen-
dc.format.extent686~693-
dc.relation.isPartOfJournal of Microbiology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAntibiotics, Antitubercular/pharmacology*-
dc.subject.MESHBase Sequence-
dc.subject.MESHDNA-Directed RNA Polymerases/genetics-
dc.subject.MESHDrug Resistance, Bacterial-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHMutation-
dc.subject.MESHMycobacterium leprae/drug effects*-
dc.subject.MESHMycobacterium leprae/genetics*-
dc.subject.MESHMycobacterium leprae/isolation & purification-
dc.subject.MESHNucleic Acid Hybridization/methods*-
dc.subject.MESHPolymerase Chain Reaction/methods*-
dc.subject.MESHRifampin/pharmacology*-
dc.subject.MESHSensitivity and Specificity-
dc.titlePerformance of PCR-reverse blot hybridization assay for detection of rifampicin-resistant Mycobacterium leprae-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학)-
dc.contributor.googleauthorHyeyoung Wang-
dc.contributor.googleauthorHyunjung Kim-
dc.contributor.googleauthorYeun Kim-
dc.contributor.googleauthorHyeeun Bang-
dc.contributor.googleauthorJong Pill Kim-
dc.contributor.googleauthorJoo Hwan Hwang-
dc.contributor.googleauthorSang Nae Cho-
dc.contributor.googleauthorTae Ue Kim-
dc.contributor.googleauthorHyeyoung Lee-
dc.identifier.doi10.1007/s12275-015-5057-9-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03824-
dc.relation.journalcodeJ01593-
dc.identifier.pmid26428919-
dc.identifier.urlhttp://link.springer.com/article/10.1007/s12275-015-5057-9-
dc.subject.keywordMycobacterium leprae-
dc.subject.keywordPCR-REBA (Reverse blot hybridization assay)-
dc.subject.keywordmolecular diagnosis-
dc.subject.keywordrifampicin-
dc.contributor.alternativeNameCho, Sang Nae-
dc.contributor.affiliatedAuthorCho, Sang Nae-
dc.rights.accessRightsnot free-
dc.citation.volume53-
dc.citation.number10-
dc.citation.startPage686-
dc.citation.endPage693-
dc.identifier.bibliographicCitationJournal of Microbiology, Vol.53(10) : 686-693, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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