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Gastrin-releasing peptide expression and its effect on the calcification of developing mouse incisor

Authors
 Dong-Joon Lee  ;  Chengri Jin  ;  Eun-Jung Kim  ;  Jong-Min Lee  ;  Han-Sung Jung 
Citation
 HISTOCHEMISTRY AND CELL BIOLOGY, Vol.144(3) : 273-279, 2015 
Journal Title
 HISTOCHEMISTRY AND CELL BIOLOGY 
ISSN
 0948-6143 
Issue Date
2015
MeSH
Ameloblasts/physiology ; Animals ; Calcification, Physiologic/genetics* ; Cells, Cultured ; Dental Enamel/metabolism ; Dentin/metabolism ; Epithelial Cells/physiology ; Gastrin-Releasing Peptide/genetics* ; Gastrin-Releasing Peptide/physiology* ; Genetic Vectors ; Incisor/growth & development* ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred ICR ; Odontoblasts/physiology ; Odontogenesis/genetics
Keywords
Gastrin-releasing peptide (GRP) ; Incisor development ; Ameloblast ; Odontoblast ; Dentin ; Enamel
Abstract
Gastrin-releasing peptide (GRP) is considered to be one of the cancer growth factors. This peptide's receptor (GRPR) is known as a G protein-coupled receptor, regulating intracellular calcium storage and releasing signals. This study is the first to investigate the function of GRP during mouse incisor development. We hypothesized that GRP is one of the factors that affects the regulation of calcification during tooth development. To verify the expression pattern of GRP, in situ hybridization was processed during incisor development. GRP was expressed at the late bell stage and hard tissue formation stage in the epithelial tissue. To identify the genuine function of GRP during incisor development, a gain-of-function analysis was performed. After GRP overexpression in culture, the phenotype of ameloblasts, odontoblasts and predentin was altered compared to control group. Moreover, enamel and dentin thickness was increased after renal capsule transplantation of GRP-overexpressed incisors. With these results, we suggest that GRP plays a significant role in the formation of enamel and dentin by regulating ameloblasts and predentin formation, respectively. Thus, GRP signaling is strongly related to calcium acquisition and secretion during mouse incisor development.
Full Text
http://link.springer.com/article/10.1007%2Fs00418-015-1335-1
DOI
10.1007/s00418-015-1335-1
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun-Jung(김은정) ORCID logo https://orcid.org/0000-0002-9515-7590
Lee, Dong Joon(이동준) ORCID logo https://orcid.org/0000-0001-6532-9729
Lee, Jong Min(이종민) ORCID logo https://orcid.org/0000-0002-9466-7644
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140827
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