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Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration

Authors
 Jung-Seok Lee  ;  Jung-Chul Park  ;  Tae-Wan Kim  ;  Byung-Joo Jung  ;  Youngseok Lee  ;  Eun-Kyung Shim  ;  Soyon Park  ;  Eun-Young Choi  ;  Kyoo-Sung Cho  ;  Chang-Sung Kim 
Citation
 Bone, Vol.78 : 34-45, 2015 
Journal Title
 Bone 
ISSN
 8756-3282 
Issue Date
2015
Abstract
Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from bone marrow of the vertebral body. The hBMSCs were cultured under either hypoxic (1% O2) or normoxic (21% O2; control) conditions and the characteristics as mesenchymal stem cells were compared. Results revealed that hypoxia reduced proliferative potential and colony-forming efficiency of hBMSCs, and significantly enhanced osteogenic and chondrogenic differentiation. The hBMSCs enhanced the regenerative potential of bone in vivo. In vitro synthesis of soluble and insoluble collagen was significantly increased in the hypoxic condition. In vivo collagen tissue regeneration was also enhanced under the hypoxic condition, with concomitant increased expressions of various subtypes of collagen and lysyl-oxidase family mRNA. MicroRNA assays revealed that miR-155-5p, which negatively regulates HIF-1α, was significantly highly expressed. These observations demonstrate that hBMSCs obtained from human vertebrae exhibit altered characteristics under hypoxic conditions, and each factor contributing to hBMSC-mediated tissue healing should be evaluated with the goal of allowing their clinical application.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140734
DOI
10.1016/j.bone.2015.04.044
Appears in Collections:
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Institute for Periodontal Tissue Regeneration (치주조직재생연구소)
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실)
Yonsei Authors
김창성(Kim, Chang Sung)
박소연(Park, So Yon)
이중석(Lee, Jung Seok)
조규성(Cho, Kyoo Sung)
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Full Text
http://www.sciencedirect.com/science/article/pii/S8756328215001659
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