0 438

Cited 25 times in

Human bone marrow stem cells cultured under hypoxic conditions present altered characteristics and enhanced in vivo tissue regeneration

Authors
 Jung-Seok Lee  ;  Jung-Chul Park  ;  Tae-Wan Kim  ;  Byung-Joo Jung  ;  Youngseok Lee  ;  Eun-Kyung Shim  ;  Soyon Park  ;  Eun-Young Choi  ;  Kyoo-Sung Cho  ;  Chang-Sung Kim 
Citation
 BONE, Vol.78 : 34-45, 2015 
Journal Title
 BONE 
ISSN
 8756-3282 
Issue Date
2015
MeSH
Adipocytes/cytology ; Adult ; Bone Marrow Cells/cytology* ; Cell Differentiation ; Cell Hypoxia ; Cell Proliferation ; Cells, Cultured ; Chondrocytes/cytology ; Chondrogenesis ; Collagen/chemistry ; Female ; Flow Cytometry ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Male ; Mesenchymal Stromal Cells/cytology* ; MicroRNAs/metabolism ; Middle Aged ; Osteogenesis ; Oxygen/chemistry ; Regeneration/physiology* ; Spine/cytology ; Stem Cells ; Wound Healing
Keywords
Hypoxia ; Mesenchymal stem cells ; Stem cell microenvironment interactions ; Tissue regeneration
Abstract
Human bone marrow mesenchymal stem cells (hBMSCs) were isolated from bone marrow of the vertebral body. The hBMSCs were cultured under either hypoxic (1% O2) or normoxic (21% O2; control) conditions and the characteristics as mesenchymal stem cells were compared. Results revealed that hypoxia reduced proliferative potential and colony-forming efficiency of hBMSCs, and significantly enhanced osteogenic and chondrogenic differentiation. The hBMSCs enhanced the regenerative potential of bone in vivo. In vitro synthesis of soluble and insoluble collagen was significantly increased in the hypoxic condition. In vivo collagen tissue regeneration was also enhanced under the hypoxic condition, with concomitant increased expressions of various subtypes of collagen and lysyl-oxidase family mRNA. MicroRNA assays revealed that miR-155-5p, which negatively regulates HIF-1α, was significantly highly expressed. These observations demonstrate that hBMSCs obtained from human vertebrae exhibit altered characteristics under hypoxic conditions, and each factor contributing to hBMSC-mediated tissue healing should be evaluated with the goal of allowing their clinical application.
Full Text
http://www.sciencedirect.com/science/article/pii/S8756328215001659
DOI
10.1016/j.bone.2015.04.044
Appears in Collections:
5. Research Institutes (연구소) > Institute for Periodontal Tissue Regeneration (치주조직재생연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Park, So Yon(박소연)
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140734
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links