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Let7a involves in neural stem cell differentiation relating with TLX level

Authors
 Juhyun Song  ;  Kyoung Joo Cho  ;  Yumi Oh  ;  Jong Eun Lee 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.462(4) : 396-401, 2015 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2015
MeSH
Animals ; Base Sequence ; Cell Differentiation/genetics* ; DNA Primers ; Female ; Mice ; Mice, Inbred ICR ; MicroRNAs/genetics ; MicroRNAs/physiology* ; Neural Stem Cells/cytology* ; Pregnancy ; Receptors, Cytoplasmic and Nuclear/metabolism* ; Reverse Transcriptase Polymerase Chain Reaction
Keywords
Let7a ; Neural stem cell ; Neuronal differentiation ; Nuclear factor TLX
Abstract
Neural stem cells (NSCs) have the potential for differentiation into neurons known as a groundbreaking therapeutic solution for central nervous system (CNS) diseases. To resolve the therapeutic efficiency of NSCs, recent researchers have focused on the study on microRNA's role in CNS. Some micro RNAs have been reported significant functions in NSC self-renewal and differentiation through the post-transcriptional regulation of neurogenesis genes. MicroRNA-Let7a (Let7a) has known as the regulator of diverse cellular mechanisms including cell differentiation and proliferation. In present study, we investigated whether Let7a regulates NSC differentiation by targeting the nuclear receptor TLX, which is an essential regulator of NSC self-renewal, proliferation and differentiation. We performed the following experiments: western blot analysis, TaqMan assay, RT-PCR, and immunocytochemistry to confirm the alteration of NSCs. Our data showed that let7a play important roles in controlling NSC fate determination. Thus, manipulating Let-7A and TLX could be a novel strategy to enhance the efficiency of NSC's neuronal differentiation for CNS disorders.
Files in This Item:
T201501700.pdf Download
DOI
10.1016/j.bbrc.2015.05.004
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Song, Ju Hyun(송주현)
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
Cho, Kyuong Joo(조경주)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/140255
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