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Allergenic characterization of a novel allergen, homologous to chymotrypsin, from german cockroach

 Kyoung Yong Jeong  ;  Mina Son  ;  Jae-Hyun Lee  ;  Chein-Soo Hong  ;  Jung-Won Park 
 Allergy Asthma & Immunology Research, Vol.7(3) : 283-289, 2015 
Journal Title
 Allergy Asthma & Immunology Research 
Issue Date
PURPOSE: Cockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease. METHODS: A cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA. RESULTS: The deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1% identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6%) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract. CONCLUSIONS: A novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6.
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5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
박중원(Park, Jung Won) ORCID logo https://orcid.org/0000-0003-0249-8749
이재현(Lee, Jae Hyun) ORCID logo https://orcid.org/0000-0002-0760-0071
정경용(Jeong, Kyoung Yong) ORCID logo https://orcid.org/0000-0001-9887-1426
홍천수(Hong, Chein Soo)
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