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Allergenic characterization of a novel allergen, homologous to chymotrypsin, from german cockroach

Authors
 Kyoung Yong Jeong  ;  Mina Son  ;  Jae-Hyun Lee  ;  Chein-Soo Hong  ;  Jung-Won Park 
Citation
 ALLERGY ASTHMA & IMMUNOLOGY RESEARCH, Vol.7(3) : 283-289, 2015 
Journal Title
ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
ISSN
 2092-7355 
Issue Date
2015
Keywords
Allergens ; German cockroach ; chymotrypsin
Abstract
PURPOSE: Cockroach feces are known to be rich in IgE-reactive components. Various protease allergens were identified by proteomic analysis of German cockroach fecal extract in a previous study. In this study, we characterized a novel allergen, a chymotrypsin-like serine protease.

METHODS: A cDNA sequence homologous to chymotrypsin was obtained by analysis of German cockroach expressed sequence tag (EST) clones. The recombinant chymotrypsins from the German cockroach and house dust mite (Der f 6) were expressed in Escherichia coli using the pEXP5NT/TOPO vector system, and their allergenicity was investigated by ELISA.

RESULTS: The deduced amino acid sequence of German cockroach chymotrypsin showed 32.7 to 43.1% identity with mite group 3 (trypsin) and group 6 (chymotrypsin) allergens. Sera from 8 of 28 German cockroach allergy subjects (28.6%) showed IgE binding to the recombinant protein. IgE binding to the recombinant cockroach chymotrypsin was inhibited by house dust mite chymotrypsin Der f 6, while it minimally inhibited the German cockroach whole body extract.

CONCLUSIONS: A novel allergen homologous to chymotrypsin was identified from the German cockroach and was cross-reactive with Der f 6.
Files in This Item:
T201501280.pdf Download
DOI
10.4168/aair.2015.7.3.283
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Lee, Jae Hyun(이재현) ORCID logo https://orcid.org/0000-0002-0760-0071
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/139998
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